Table 1. Agonist-induced [ 14 C]5-HT... | American Society of Hematology

Table 1.

Agonist-induced [¹⁴C]5-HT secretion and aggregation in washed platelets prepared from normal and patient blood

	[¹⁴C]5-HT secretion, % of total cpm			% maximum aggregation
Agonist	N	P	N	P	N	P
10 μM ADP	2	0	54	2	31	2
2 μg/mL collagen	44	6	73	12	29	6
20 μg/mL collagen	65	57	78	68	30	18
0.05 U/mL thrombin	71	71	68	81	35	14
0.2 U/mL thrombin	78	76	76	71	30	14
3 μM U46619	7	0	15	8	15	4
100 nM PMA	12	10	42	39	15	15

	[¹⁴C]5-HT secretion, % of total cpm			% maximum aggregation
Agonist	N	P	N	P	N	P
10 μM ADP	2	0	54	2	31	2
2 μg/mL collagen	44	6	73	12	29	6
20 μg/mL collagen	65	57	78	68	30	18
0.05 U/mL thrombin	71	71	68	81	35	14
0.2 U/mL thrombin	78	76	76	71	30	14
3 μM U46619	7	0	15	8	15	4
100 nM PMA	12	10	42	39	15	15

Washed platelets labeled with [¹⁴C]5-HT were prepared as described in “Patient, materials, and methods.” Platelets were incubated with agonists for 3 minutes followed by measurement of aggregation and percent [¹⁴C]5-HT secretion. With secretion studies, release from unstimulated platelets was corrected from subsequent values. The results are expressed as a percent of total counts per minute incorporated. Aggregation was measured as maximum percent light transmission change relative to platelets in resuspension buffer (RSB) versus RSB alone. Initial rate of aggregation was calculated from the initial slope of the light transmittance change versus time and expressed as arbitary units. The values shown are the averages from 2 separate experiments.

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