Agonist-induced [3H]arachidonate release and TxB2 generation in washed platelets prepared from normal and patient blood
. | [3H]arachidonate release, % of total cpm . | . | TxB2, ng/108 platelets . | . | ||
|---|---|---|---|---|---|---|
| Agonist . | N . | P . | N . | P . | ||
| Resting | 2.56 | 2.24 | 0.45 | 0.35 | ||
| 0.2 U/mL thrombin | 14.21 | 13.46 | 65 | 73 | ||
| 20 μg/mL collagen | 11.21 | 12.15 | 75 | 73 | ||
. | [3H]arachidonate release, % of total cpm . | . | TxB2, ng/108 platelets . | . | ||
|---|---|---|---|---|---|---|
| Agonist . | N . | P . | N . | P . | ||
| Resting | 2.56 | 2.24 | 0.45 | 0.35 | ||
| 0.2 U/mL thrombin | 14.21 | 13.46 | 65 | 73 | ||
| 20 μg/mL collagen | 11.21 | 12.15 | 75 | 73 | ||
Washed platelets were either labeled with [3H]arachidonic acid or left unlabeled for TxB2 studies, as described in “Patient, materials, and methods.” All values were measured 3 minutes after agonist addition. TxB2 released into the supernatant was measured using a TxB2 enzyme immunoassay kit from Amersham Life Science. The values shown are the averages from 2 separate experiments.