Vasopermeability and cytokine activity of chTNT-3/IL-2 WT and IL-2 analog fusion proteins
Molecule . | Vasopermeability, % ± SD . | HT-2 proliferation* . |
|---|---|---|
| chTNT-3 | 0 ± 5 | — |
| chTNT-3/IL-2 WT | 100 ± 15 | ++++ |
| chTNT-3/Asp20Lys | -28 ± 6 | — |
| chTNT-3/Arg38Gly | -7 ± 15 | + |
| chTNT-3/Arg38Trp | 4 ± 16 | +++ |
| chTNT-3/Arg38Glu | -5 ± 6 | — |
| chTNT-3/Arg38Tyr | 42 ± 8 | ++ |
| chTNT-3/Met39Val | 99 ± 27 | + |
| chTNT-3/Met39Leu | 52 ± 23 | + |
| chTNT-3/Phe42Lys | 97 ± 31 | + |
| chTNT-3/His55Tyr | -6 ± 6 | + |
| chTNT-3/Asn8Arg | 98 ± 4 | +++ |
Molecule . | Vasopermeability, % ± SD . | HT-2 proliferation* . |
|---|---|---|
| chTNT-3 | 0 ± 5 | — |
| chTNT-3/IL-2 WT | 100 ± 15 | ++++ |
| chTNT-3/Asp20Lys | -28 ± 6 | — |
| chTNT-3/Arg38Gly | -7 ± 15 | + |
| chTNT-3/Arg38Trp | 4 ± 16 | +++ |
| chTNT-3/Arg38Glu | -5 ± 6 | — |
| chTNT-3/Arg38Tyr | 42 ± 8 | ++ |
| chTNT-3/Met39Val | 99 ± 27 | + |
| chTNT-3/Met39Leu | 52 ± 23 | + |
| chTNT-3/Phe42Lys | 97 ± 31 | + |
| chTNT-3/His55Tyr | -6 ± 6 | + |
| chTNT-3/Asn8Arg | 98 ± 4 | +++ |
Stimulation of HT-2 proliferation expressed as percentage of wild-type activity: — indicates no activity, +, less than 25% activity, ++, 25%-50% activity, +++, 50%-75% activity, ++++, 75%-100%.