LacZ reporter activation in cotransformants of BD-FANCG with AD-FANC constructs
Full-length protein fused to GAL4-AD . | β-Galactosidase activity* . |
|---|---|
| No insert | 41.2 ± 1.5 |
| FANCA | 100.1 ± 5.2 |
| FANCC | 42.8 ± 3.3 |
| FANCD2 | 41.0 ± 2.6 |
| FANCE | 44.5 ± 6.9 |
| FANCF | 23.2 ± 1.6 |
| FANCG | 42.7 ± 1.4 |
Full-length protein fused to GAL4-AD . | β-Galactosidase activity* . |
|---|---|
| No insert | 41.2 ± 1.5 |
| FANCA | 100.1 ± 5.2 |
| FANCC | 42.8 ± 3.3 |
| FANCD2 | 41.0 ± 2.6 |
| FANCE | 44.5 ± 6.9 |
| FANCF | 23.2 ± 1.6 |
| FANCG | 42.7 ± 1.4 |
Units of β-galactosidase activity in the liquid ONPG assay were calculated as 1000 × optical density at 420 nm (OD420) divided by the product of the volume (milliliter) × time (minutes) × OD600.