Table 2.

Intracellular calcein fluorescence, estimated calcein, and labile iron (Fe2+) values in erythrocytes


Erythrocyte hemoglobin genotype

Absolute fluorescence, au

Fractional rise in fluorescence, ΔF

RBC calcein [CA]t, μM

Erythrocyte labile iron (II), μM
HbSS RBC, n = 29   38.70 ± 23.30  0.22 ± 0.08  2.03 ± 1.02§  0.56 ± 0.35§ 
HbSC, Sβthal RBC, n = 5   36.55 ± 9.17  0.26 ± 0.05*  2.71 ± 0.55   0.81 ± 0.24 
HbAS RBC, n = 7   34.14 ± 13.44  0.30 ± 0.13   3.02 ± 1.23   1.09 ± 0.70  
HbAA RBC, n = 17
 
55.50 ± 27.13
 
0.32 ± 0.11
 
3.24 ± 1.12
 
1.25 ± 0.65
 

Erythrocyte hemoglobin genotype

Absolute fluorescence, au

Fractional rise in fluorescence, ΔF

RBC calcein [CA]t, μM

Erythrocyte labile iron (II), μM
HbSS RBC, n = 29   38.70 ± 23.30  0.22 ± 0.08  2.03 ± 1.02§  0.56 ± 0.35§ 
HbSC, Sβthal RBC, n = 5   36.55 ± 9.17  0.26 ± 0.05*  2.71 ± 0.55   0.81 ± 0.24 
HbAS RBC, n = 7   34.14 ± 13.44  0.30 ± 0.13   3.02 ± 1.23   1.09 ± 0.70  
HbAA RBC, n = 17
 
55.50 ± 27.13
 
0.32 ± 0.11
 
3.24 ± 1.12
 
1.25 ± 0.65
 

Results are mean ± standard deviation. au indicates arbitary units.

*

P < .1 compared with Hb AA group.

P < .05 compared with HbAA group.

P < .01 compared with HbAA group.

§

P < .001 compared with HbAA group.

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