Intracellular calcein fluorescence, estimated calcein, and labile iron values in hemoglobin AA and hemoglobin SS erythrocytes measured using BIP or SIH
. | Absolute fluorescence rise, au . | . | . | Fractional fluorescence rise, ΔF . | . | . | Erythrocyte, calcein [CA]t, μM . | . | . | Erythrocyte labile iron pool, μM . | . | . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Erythrocyte hemoglobin genotype . | BIP . | SIH . | P* . | BIP . | SIH . | P* . | BIP . | SIH . | P* . | BIP . | SIH . | P* . | ||||||||
| HbSS erythrocytes, n = 12 | 25.49 ± 7.67† | 75.27 ± 16.82† | < .001 | 0.18 ± 0.03‡ | 0.40 ± 0.03† | < .001 | 3.24 ± 0.62§ | 3.95 ± 0.93† | < .037 | 0.65 ± 0.16∥ | 1.75 ± 0.41† | < .001 | ||||||||
| HbAA erythrocytes, n = 10 | 32.60 ± 10.09 | 65.96 ± 15.54 | < .001 | 0.25 ± 0.06 | 0.40 ± 0.10 | < .003 | 3.94 ± 0.54 | 4.48 ± 1.16 | .202 | 1.09 ± 0.36 | 2.14 ± 0.93 | < .004 | ||||||||
. | Absolute fluorescence rise, au . | . | . | Fractional fluorescence rise, ΔF . | . | . | Erythrocyte, calcein [CA]t, μM . | . | . | Erythrocyte labile iron pool, μM . | . | . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Erythrocyte hemoglobin genotype . | BIP . | SIH . | P* . | BIP . | SIH . | P* . | BIP . | SIH . | P* . | BIP . | SIH . | P* . | ||||||||
| HbSS erythrocytes, n = 12 | 25.49 ± 7.67† | 75.27 ± 16.82† | < .001 | 0.18 ± 0.03‡ | 0.40 ± 0.03† | < .001 | 3.24 ± 0.62§ | 3.95 ± 0.93† | < .037 | 0.65 ± 0.16∥ | 1.75 ± 0.41† | < .001 | ||||||||
| HbAA erythrocytes, n = 10 | 32.60 ± 10.09 | 65.96 ± 15.54 | < .001 | 0.25 ± 0.06 | 0.40 ± 0.10 | < .003 | 3.94 ± 0.54 | 4.48 ± 1.16 | .202 | 1.09 ± 0.36 | 2.14 ± 0.93 | < .004 | ||||||||
Results are mean ± standard deviation. P value determined at .05 level comparing the mean values of each variable for hemoglobin SS versus hemoglobin AA. BIP indicates 2′2′-bipyridyl, iron (Fe2+) chelator; SIH, salicylaldehyde isonicotinoyl hydrazone, iron (Fe3+) chelator.
P value, BIP vs SIH for HbSS or HbAA erythrocytes.
P > .05 compared with HbAA group.
P < .05 compared with HbAA group.
P < .01 compared with HbAA group.
P < .001 compared with HbAA group.