Average intracellullar amounts per cell of hemoglobin iron, cytosolic labile iron, and membrane-associated iron in hemoglobin AA and hemoglobin SS erythrocytes
Variable . | HbAA, n = 6 . | HbSS, n = 6 . | P* . |
|---|---|---|---|
| Hemoglobin iron, × 10−4 fmol/cell | 17 873 ± 1 348 | 18 763 ± 3 207 | .65 |
| RBC labile Fe2+ (with calcein & BIP), × 10−4 fmol/cell | 1.08 ± 0.34 | 0.66 ± 0.22 | .03 |
| RBC total labile Fe2+ and Fe3+ (with calcein & SIH), × 10−4 fmol/cell | 1.58 ± 0.71 | 1.78 ± 0.48 | .59 |
| RBC membrane iron, × 10−4 fmol/cell | 0.0004 ± 0.0001 | 0.0016 ± 0.0009 | .01 |
Variable . | HbAA, n = 6 . | HbSS, n = 6 . | P* . |
|---|---|---|---|
| Hemoglobin iron, × 10−4 fmol/cell | 17 873 ± 1 348 | 18 763 ± 3 207 | .65 |
| RBC labile Fe2+ (with calcein & BIP), × 10−4 fmol/cell | 1.08 ± 0.34 | 0.66 ± 0.22 | .03 |
| RBC total labile Fe2+ and Fe3+ (with calcein & SIH), × 10−4 fmol/cell | 1.58 ± 0.71 | 1.78 ± 0.48 | .59 |
| RBC membrane iron, × 10−4 fmol/cell | 0.0004 ± 0.0001 | 0.0016 ± 0.0009 | .01 |
Results are mean ± standard deviation. BIP indicates 2′2′-bipyridyl, iron (Fe2+) chelator; SIH, salicylaldehyde isonicotinoyl hydrazone, iron (Fe3+) chelator.
P value HbSS compared with HbAA group.