Cell cycle-dependent up-regulation of Fas expression on hematopoletic progenitor/stem cells
. | . | Cell cycle distribution, % (SEM) . | . | . | ||
|---|---|---|---|---|---|---|
| Cell treatment and Fas expression . | % of total CD34+ cells . | G0 . | G1 . | S/G2/M . | ||
| Freshly isolated | ||||||
| Fas+ | 14 | 53 (5) | 43 (6) | 3 (1) | ||
| Fas− | 86 | 69 (5) | 30 (5) | 1 (1) | ||
| SFT3, 5 days | ||||||
| Fas+ | 45 | 5 (1) | 44 (4) | 49 (3) | ||
| Fas− | 55 | 5 (2) | 71 (3) | 23 (2) | ||
. | . | Cell cycle distribution, % (SEM) . | . | . | ||
|---|---|---|---|---|---|---|
| Cell treatment and Fas expression . | % of total CD34+ cells . | G0 . | G1 . | S/G2/M . | ||
| Freshly isolated | ||||||
| Fas+ | 14 | 53 (5) | 43 (6) | 3 (1) | ||
| Fas− | 86 | 69 (5) | 30 (5) | 1 (1) | ||
| SFT3, 5 days | ||||||
| Fas+ | 45 | 5 (1) | 44 (4) | 49 (3) | ||
| Fas− | 55 | 5 (2) | 71 (3) | 23 (2) | ||
Freshly isolated or cultured (SFT3 for 5 days) CD34+ CB cells were sorted into CD34+ Fas+ and CD34+ Fas− subpopulations and investigated for cell cycle distribution following staining with 7-AAD and anti-Ki-67 (“Materials and methods”). Results are from 3 separate experiments for freshly isolated and cultured cells; mean values (SEM).