In vitro cycling LTC-ICs up-regulate Fas expression
Experiment, expansion, and sorted cells . | % of total Lin− cells . | LTC-CFCs (replicate values)/seeded cells . |
|---|---|---|
| Exp 1, 28-fold expansion | ||
| BM Lin−Fas− | 34 | 13 (3-23)/7000 |
| BM Lin−Fas+ | 66 | 134 (110-158)/7000 |
| Exp 2, 20-fold expansion | ||
| BM Lin−Fas− | 48 | 5 (4-7)/5000 |
| BM Lin−Fas+ | 52 | 56 (37-67)/5000 |
| Exp 3, 14-fold expansion | ||
| BM Lin−Fas− | 35 | 6 (4-9)/3500 |
| BM Lin−Fas+ | 65 | 52 (37-52)/3500 |
| Exp 4, 16-fold expansion | ||
| CB Lin−Fas− | 14 | 7 (0-5-17)/2400 |
| CB Lin−Fas+ | 86 | 27 (13-15-25)/2400 |
| Exp 5, 19-fold expansion | ||
| CB Lin− | 100 | 40 (23-23-75)/2850 |
| CB Lin−Fas+ | 70 | 140 (45-143-233)/2850 |
Experiment, expansion, and sorted cells . | % of total Lin− cells . | LTC-CFCs (replicate values)/seeded cells . |
|---|---|---|
| Exp 1, 28-fold expansion | ||
| BM Lin−Fas− | 34 | 13 (3-23)/7000 |
| BM Lin−Fas+ | 66 | 134 (110-158)/7000 |
| Exp 2, 20-fold expansion | ||
| BM Lin−Fas− | 48 | 5 (4-7)/5000 |
| BM Lin−Fas+ | 52 | 56 (37-67)/5000 |
| Exp 3, 14-fold expansion | ||
| BM Lin−Fas− | 35 | 6 (4-9)/3500 |
| BM Lin−Fas+ | 65 | 52 (37-52)/3500 |
| Exp 4, 16-fold expansion | ||
| CB Lin−Fas− | 14 | 7 (0-5-17)/2400 |
| CB Lin−Fas+ | 86 | 27 (13-15-25)/2400 |
| Exp 5, 19-fold expansion | ||
| CB Lin− | 100 | 40 (23-23-75)/2850 |
| CB Lin−Fas+ | 70 | 140 (45-143-233)/2850 |
CD34+ CB or BM cells were cultured in SF medium supplemented with SFT3 for 5 days, at which time Lin−Fas− (or total Lin− cells; experiment 5) and Lin−Fas− cells were sorted on a FACSVantage and evaluated for LTC-CFC activity. The expansion equivalents (EEs) of 150 CB or 250 BM CD34+ cells were always investigated. Data shown are mean values, with replicate values in parentheses.
Exp indicates experiment.