Fas-expressing SRCs are resistant to Fas activation
. | Without CH11 . | . | . | With CH11 . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Experiment . | Fold expansion . | Human engraftment, % (replicate %) . | Positive mice . | Fold expansion . | Human engraftment, % (replicate %) . | Positive mice . | ||||
| Exp 1 | 33 | 23 (5-12-18-20-58) | 5/5 | 32 | 22 (0-13-17-38-44) | 4/5 | ||||
| Exp 2 | 21 | 1 (0.4-1-1-3) | 4/4 | 18 | 5 (0-1-3-5-16) | 4/5 | ||||
| Exp 3 | 158 | 11 (3-4-6-10-31) | 5/5 | 160 | 30 (14-25-30-41-64) | 5/5 | ||||
. | Without CH11 . | . | . | With CH11 . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Experiment . | Fold expansion . | Human engraftment, % (replicate %) . | Positive mice . | Fold expansion . | Human engraftment, % (replicate %) . | Positive mice . | ||||
| Exp 1 | 33 | 23 (5-12-18-20-58) | 5/5 | 32 | 22 (0-13-17-38-44) | 4/5 | ||||
| Exp 2 | 21 | 1 (0.4-1-1-3) | 4/4 | 18 | 5 (0-1-3-5-16) | 4/5 | ||||
| Exp 3 | 158 | 11 (3-4-6-10-31) | 5/5 | 160 | 30 (14-25-30-41-64) | 5/5 | ||||
Results are presented as percentage of human engraftment of mice receiving transplants with the expansion equivalent (EE) of 100 000 (exp 1) or 50 000 (exp 2 and exp 3) CD34+ CB cells cultured for 5 (exp 1 and exp 2) or 7 (exp 3) days in SF medium supplemented with SFT3 in the absence or presence of CH11 (1 μg/mL; mean percentages are presented with replicate percentages in parentheses). Also shown are fractions of mice positive for human reconstitution with both B and myeloid cells (“Materials and methods”).
Abbreviations are explained in Table 3.