Angiotensin II enhances the extracellular release of H2O2 by human neutrophils
. | H2O2, nmol/106 cells × min . | . | |
|---|---|---|---|
| Additions to cells . | Resting . | IFN-γ/LPS treated . | |
| None | 0.37 ± 0.02 | 0.53 ± 0.01 | |
| Ang (100) | 0.59 ± 0.01 | 0.82 ± 0.01 | |
| Ang (1000) | 1.00 ± 0.02 | 1.30 ± 0.02 | |
. | H2O2, nmol/106 cells × min . | . | |
|---|---|---|---|
| Additions to cells . | Resting . | IFN-γ/LPS treated . | |
| None | 0.37 ± 0.02 | 0.53 ± 0.01 | |
| Ang (100) | 0.59 ± 0.01 | 0.82 ± 0.01 | |
| Ang (1000) | 1.00 ± 0.02 | 1.30 ± 0.02 | |
Neutrophils (9 × 106 cells/mL) were preincubated alone (resting) or with IFN-γ/LPS (100 U/mL and 1 μg/mL, respectively) for 60 minutes and were further treated with Ang II for 2 hours at 37°C. The amount of H2O2 released was measured by the phenol-red method. Data are expressed as the means ± SEMs of 3 separate experiments