Table 1. Characteristics of patient... | American Society of Hematology

Table 1.

Characteristics of patient samples

Collection ID	Diagnosis	Subtype	Sample type	Estimated blasts, %^*	Flt 3 ITD†
53	AML	M2	Pheresis	98	ND
55	AML	M2	PB	78	ND
61	AML	M2	PB	17	-
72	AML	With dysplasia	PB	53	-
75	ALL	Precursor-B cell	PB	74	ND
78	AML	M2	Pheresis	98	ND
79	AML	M4	Pheresis	70	ND
80	AML	M5	PB	53	ND
82	CML	Lymphoid blast crisis	Pheresis	89	ND
85	AML	M2	BM	90	-
87‡	AML	Biphenotypic	PB	73	+
88‡	AML	Biphenotypic	BM	73	+
109	AML	M1	Pheresis	89	+
125	AML	M1	PB	80	+
128	AML	M2	Pheresis	55	-
132	AML	With dysplasia	PB	76	-
134	AML	§	Pheresis	78	+
144	AML	With dysplasia	Pheresis	98	-
146	AML	M2	Pheresis	97	ND
147	AML	With dysplasia	PB	58	-
149	AML	M1	BM	91	+
150	AML	M2	PB	88	ND
151	AML	M4	PB	22	-
152	AML	M3	PB	96	+
159	AML	M2	PB	59	-

Collection ID	Diagnosis	Subtype	Sample type	Estimated blasts, %^*	Flt 3 ITD†
53	AML	M2	Pheresis	98	ND
55	AML	M2	PB	78	ND
61	AML	M2	PB	17	-
72	AML	With dysplasia	PB	53	-
75	ALL	Precursor-B cell	PB	74	ND
78	AML	M2	Pheresis	98	ND
79	AML	M4	Pheresis	70	ND
80	AML	M5	PB	53	ND
82	CML	Lymphoid blast crisis	Pheresis	89	ND
85	AML	M2	BM	90	-
87‡	AML	Biphenotypic	PB	73	+
88‡	AML	Biphenotypic	BM	73	+
109	AML	M1	Pheresis	89	+
125	AML	M1	PB	80	+
128	AML	M2	Pheresis	55	-
132	AML	With dysplasia	PB	76	-
134	AML	§	Pheresis	78	+
144	AML	With dysplasia	Pheresis	98	-
146	AML	M2	Pheresis	97	ND
147	AML	With dysplasia	PB	58	-
149	AML	M1	BM	91	+
150	AML	M2	PB	88	ND
151	AML	M4	PB	22	-
152	AML	M3	PB	96	+
159	AML	M2	PB	59	-

Blast percentages are determined in clinical laboratories based on differential of peripheral blood or bone marrow aspiration prior to Ficoll sedimentation. Numbers may underestimate actual percent blasts in mononuclear cell fraction used for experiments

PB indicates peripheral blood; BM, bone marrow; ND, not done; -, negative; and +, positive

†

Analysis of D835 mutation also was performed, but none of these samples was positive. Although 7 of 16 samples are positive for Flt3 ITDs in this group, this was not representative of the tissue bank in which 11 of 57 (18%) of samples are positive

‡

Samples 87 and 88 are peripheral blood and bone marrow, respectively, from the same patient

Patient presented with an elevated white blood cell count and clinical evidence of leukostasis. Diagnosis of acute myeloid leukemia was made based on morphologic appearance of blast cells, but the patient died before these blasts could be immunophenotypically and cytochemically characterized, precluding a definitive pathologic subclassification

View Large

This Feature Is Available To Subscribers Only