Actin polymerization in chemokine receptor-transfected cells and monocytes
Receptor . | Selective agonist . | Eotaxin-3 . | NC . |
|---|---|---|---|
| CCR1 | 1.68±0.16* | 1.05±0.02 | 1.0 |
| CCR2 | 1.31±0.02* | 0.95±0.01 | 1.0 |
| CCR5 | 1.32±0.05† | 0.95±0.03 | 1.0 |
| CCR8 | 1.65±0.13* | 0.97±0.01 | 1.0 |
| CXCR4 | 1.24±0.05† | 1.01±0.02 | 1.0 |
| Monocytes | 3.10±0.08 | 1.90±0.1 | 1.0 |
Receptor . | Selective agonist . | Eotaxin-3 . | NC . |
|---|---|---|---|
| CCR1 | 1.68±0.16* | 1.05±0.02 | 1.0 |
| CCR2 | 1.31±0.02* | 0.95±0.01 | 1.0 |
| CCR5 | 1.32±0.05† | 0.95±0.03 | 1.0 |
| CCR8 | 1.65±0.13* | 0.97±0.01 | 1.0 |
| CXCR4 | 1.24±0.05† | 1.01±0.02 | 1.0 |
| Monocytes | 3.10±0.08 | 1.90±0.1 | 1.0 |
Relative F-actin content was measured in monocytes and single-receptor transfectants stimulated with 100 nM of the selective agonists (MIP-1α/CCR1, MCP-1/CCR2, MIP-1β/CCR5, I-309/CCR8, SDF-1α/CXCR4, MCP-1/monocytes), 300 nM eotaxin-3, or buffer (NC) for 10 seconds. Differences between unstimulated and agonist-stimulated single receptor-transfected cells are statistically significant. In chemokine receptor-transfected cells, eotaxin-3 response does not significantly differ from the controls. Differences between unstimulated and MCP-1- or eotaxin-3-stimulated monocytes are statistically significant (P < .02)
P < .05
P < .01