Primers and probes for species-specific adenovirus detection by real-time PCR
Virus type . | Target . | Amplicon length, bp . | Oligonucleotide sequence, 5′-3′, forward, probe, reverse . | Concentration, nM . | Nucleotide position . | GenBank Accession no. . |
|---|---|---|---|---|---|---|
| AdV A | Hexon gene | 135 | GGK CTG GTG CAA TTC GCC | 300 | 17818-17835 | X73487 |
| CCA CGG ACA CCT ACT TCA CCC TGG G | 200 | 17840-17864 | ||||
| CAC GGG CAC AAA ACG CA | 300 | 17936-17952 | ||||
| AdV B (B + E) | Hexon gene | 138 | CGC CGG ACA GGA TGC TT | 900 | 45-61 | X76549 |
| AGT CCG GGT CTG GTG CAG TTC GCC | 200 | 73-96 | ||||
| CTA CGG TCG GTG GTC AC | 900 | 166-182 | ||||
| AdV C | Hexon gene | 138 | ACC TGG GCC AAA ACC TTC TC | 300 | 2884-2903 | J01966 |
| AAC TCC GCC CAC GCG CTA GA | 200 | 2910-2929 | ||||
| CGT CCA TGG GAT CCA CCT C | 900 | 2940-2958 | ||||
| AdV D | VA RNA gene | 143 | AAA AAC GAA AGC GGT TGA GC | 300 | 2-21 | U10675 |
| CCA ATA CCA CGT TAG TCG CGG CT | 200 | 104-126 | ||||
| CGG GTC GAG ACG GGA GT | 50 | 128-144 | ||||
| AdV E | Hexon gene | 75 | CAA CAC CTA CTC GTA CAA AGT GCG | 900 | 225-248 | X84646 |
| CGC CCA CGG CCA GCG TGT | 200 | 251-268 | ||||
| TAG GTG CTG GCC ATG TCC A | 300 | 281-299 | ||||
| AdV F | Hexon gene | 128 | GCA GGA CGC CTC GGA GTA | 300 | 268-285 | D13781 |
| TAC TTC AGC CTG GGG AAC AAG TTC AGA AA | 200 | 329-357 | ||||
| TGT CTG TGG TTA CAT CGT GGG T | 900 | 374-395 |
Virus type . | Target . | Amplicon length, bp . | Oligonucleotide sequence, 5′-3′, forward, probe, reverse . | Concentration, nM . | Nucleotide position . | GenBank Accession no. . |
|---|---|---|---|---|---|---|
| AdV A | Hexon gene | 135 | GGK CTG GTG CAA TTC GCC | 300 | 17818-17835 | X73487 |
| CCA CGG ACA CCT ACT TCA CCC TGG G | 200 | 17840-17864 | ||||
| CAC GGG CAC AAA ACG CA | 300 | 17936-17952 | ||||
| AdV B (B + E) | Hexon gene | 138 | CGC CGG ACA GGA TGC TT | 900 | 45-61 | X76549 |
| AGT CCG GGT CTG GTG CAG TTC GCC | 200 | 73-96 | ||||
| CTA CGG TCG GTG GTC AC | 900 | 166-182 | ||||
| AdV C | Hexon gene | 138 | ACC TGG GCC AAA ACC TTC TC | 300 | 2884-2903 | J01966 |
| AAC TCC GCC CAC GCG CTA GA | 200 | 2910-2929 | ||||
| CGT CCA TGG GAT CCA CCT C | 900 | 2940-2958 | ||||
| AdV D | VA RNA gene | 143 | AAA AAC GAA AGC GGT TGA GC | 300 | 2-21 | U10675 |
| CCA ATA CCA CGT TAG TCG CGG CT | 200 | 104-126 | ||||
| CGG GTC GAG ACG GGA GT | 50 | 128-144 | ||||
| AdV E | Hexon gene | 75 | CAA CAC CTA CTC GTA CAA AGT GCG | 900 | 225-248 | X84646 |
| CGC CCA CGG CCA GCG TGT | 200 | 251-268 | ||||
| TAG GTG CTG GCC ATG TCC A | 300 | 281-299 | ||||
| AdV F | Hexon gene | 128 | GCA GGA CGC CTC GGA GTA | 300 | 268-285 | D13781 |
| TAC TTC AGC CTG GGG AAC AAG TTC AGA AA | 200 | 329-357 | ||||
| TGT CTG TGG TTA CAT CGT GGG T | 900 | 374-395 |