Frequency and total number of Hmgb3+ and Hmgb3- lymphoid and myeloid CFU-C
. | . | . | CMP . | . | . | . | |||
|---|---|---|---|---|---|---|---|---|---|
| . | CLP . | . | FcγRII/IIILO . | . | CD34+ . | . | |||
. | Hmgb3+ . | Hmgb3- . | Hmgb3+ . | Hmgb3- . | Hmgb3+ . | Hmgb3- . | |||
| Total cells/hind limb | 646.2 | 1747.4 | 4255.1 | 2621.6 | 2685.5 | 908.3 | |||
| CFU-pre-B | |||||||||
| CFU-pre-B/500 CLP (counted colonies) | 42.0 ± 6.8 (294) | 8.3 ± 2.5 (66) | 0 | 0 | 0 | 0 | |||
| Total CLP-derived CFU-pre-B/hind limb | 54.2 | 29.0 | 0 | 0 | 0 | 0 | |||
| CFU-GM | |||||||||
| CFU-GM/500 cells (counted colonies) | 0 | 0 | 11.5 ± 1.9 (69) | 21.0 ± 3.0 (126) | 69.0 ± 4.7 (345) | 72.4 ± 6.2 (362) | |||
| Total CMP-derived CFU-GM/hind limb | 0 | 0 | 97.9 | 110.1 | 370.6 | 131.5 | |||
| BFU-E | |||||||||
| BFU-E/500 cells (counted colonies) | 0 | 0 | 11.5 ± 1.6 (69) | 3.6 ± 1.2 (22) | 21.5 ± 2.1 (107) | 11.4 ± 2.1 (57) | |||
| Total CMP-derived BFU-E/hind limb | 0 | 0 | 97.9 | 18.9 | 115.5 | 20.7 | |||
| CFU-GEMM | |||||||||
| CFU-GEMM/500 cells (counted colonies) | 0 | 0 | 1.3 ± 1.0 (8) | 4.2 ± 1.2 (25) | 7.8 ± 1.7 (40) | 17.2 ± 2.6 (86) | |||
| Total CMP-derived CFU-GEMM/hind limb | 0 | 0 | 11.1 | 22.0 | 41.9 | 31.2 | |||
. | . | . | CMP . | . | . | . | |||
|---|---|---|---|---|---|---|---|---|---|
| . | CLP . | . | FcγRII/IIILO . | . | CD34+ . | . | |||
. | Hmgb3+ . | Hmgb3- . | Hmgb3+ . | Hmgb3- . | Hmgb3+ . | Hmgb3- . | |||
| Total cells/hind limb | 646.2 | 1747.4 | 4255.1 | 2621.6 | 2685.5 | 908.3 | |||
| CFU-pre-B | |||||||||
| CFU-pre-B/500 CLP (counted colonies) | 42.0 ± 6.8 (294) | 8.3 ± 2.5 (66) | 0 | 0 | 0 | 0 | |||
| Total CLP-derived CFU-pre-B/hind limb | 54.2 | 29.0 | 0 | 0 | 0 | 0 | |||
| CFU-GM | |||||||||
| CFU-GM/500 cells (counted colonies) | 0 | 0 | 11.5 ± 1.9 (69) | 21.0 ± 3.0 (126) | 69.0 ± 4.7 (345) | 72.4 ± 6.2 (362) | |||
| Total CMP-derived CFU-GM/hind limb | 0 | 0 | 97.9 | 110.1 | 370.6 | 131.5 | |||
| BFU-E | |||||||||
| BFU-E/500 cells (counted colonies) | 0 | 0 | 11.5 ± 1.6 (69) | 3.6 ± 1.2 (22) | 21.5 ± 2.1 (107) | 11.4 ± 2.1 (57) | |||
| Total CMP-derived BFU-E/hind limb | 0 | 0 | 97.9 | 18.9 | 115.5 | 20.7 | |||
| CFU-GEMM | |||||||||
| CFU-GEMM/500 cells (counted colonies) | 0 | 0 | 1.3 ± 1.0 (8) | 4.2 ± 1.2 (25) | 7.8 ± 1.7 (40) | 17.2 ± 2.6 (86) | |||
| Total CMP-derived CFU-GEMM/hind limb | 0 | 0 | 11.1 | 22.0 | 41.9 | 31.2 | |||
Total cells is the summation of the number of cells with the CLP or CMP phenotypes that were recovered after FACS sorting from all experiments divided by the number of hind limbs. Cells with the CLP phenotype were estimated to be 0.01% of whole bone marrow per hind limb (Figure 5). Cells with either CMP phenotype were estimated to be 0.008% of whole bone marrow per hind limb (Figure 6). Numbers in parentheses indicate total number of colonies counted. Colony frequencies were determined by dividing the total number of colonies counted by the total number of cells plated and normalizing to number of colonies per 500 cells. Total progenitor-derived CFU-C per hind limb was determined by the formula [frequency] × [total cells/hind limb].