Reduction of plasma autoantibodies against GPIb/IX and GPIIb/IIIa after absorption with platelets
. | Plasma autoantibodies after treatment . | . | |
|---|---|---|---|
| Plasma autoantibodies before treatment . | Autoantibodies completely removed . | Autoantibodies partially removed . | |
| Against GPIb/IX | |||
| 2 IgG | 1 IgG | 1 IgG | |
| 14 IgM | 4 IgM | 10 IgM | |
| Against GPIIb/IIIa | |||
| 4 IgG | 2 IgG | 2 IgG | |
| 3 IgM | 1 IgM | 2 IgM | |
. | Plasma autoantibodies after treatment . | . | |
|---|---|---|---|
| Plasma autoantibodies before treatment . | Autoantibodies completely removed . | Autoantibodies partially removed . | |
| Against GPIb/IX | |||
| 2 IgG | 1 IgG | 1 IgG | |
| 14 IgM | 4 IgM | 10 IgM | |
| Against GPIIb/IIIa | |||
| 4 IgG | 2 IgG | 2 IgG | |
| 3 IgM | 1 IgM | 2 IgM | |
There were 18 ITP plasmas (group IIb/IIIa/Ib, n = 4; group IIb/IIIa, n = 3; group Ib, n = 11). Patient plasma (0.5 mL) was mixed with the washed platelets (0.9 to 1.5 × 1010/mL plasma) and incubated at 4°C for 1 hour. After centrifugation at 3000g for 5 minutes, the supernatant plasma was again absorbed with fresh platelets at 4°C for 1 to 1.5 hours. The absorbed plasma was then analyzed by ELISA for the presence of autoantibodies on the following day