Table 1. Sequences of cell-permeable α 2... | American Society of Hematology

Table 1.

Sequences of cell-permeable α₂ or β₁ cytoplasmic tail peptides or control peptides

	Signal sequence	Cytoplasmic tail sequence
Signal-α₂ cytoplasmic tail	VTVLALGALAGVGVG	KLGFFKRKYEKMTKNPDEIDETTELSS
α₂ cytoplasmic tail		KLGFFKRKYEKMTKNPDEIDETTELSS
Signal-α₂ without GFFKR	VTVLALGALAGVGVG	KL_____KYEKMTKNPDEIDETTELSS
Signal-KLGFFKR	VTVLALGALAGVGVG	KLGFFKR
Signal-scrambled α₂ tail	VTVLALGALAGVGVG	TKSKYNEGSELIKTLKMTDFFREDKEP
Signal-β₁ cytoplasmic tail	VTVLALGALAGVGVG	NPIYKSAVTTVVNPKYEGK
β₁ cytoplasmic tail		NPIYKSAVTTVVNPKYEGK

	Signal sequence	Cytoplasmic tail sequence
Signal-α₂ cytoplasmic tail	VTVLALGALAGVGVG	KLGFFKRKYEKMTKNPDEIDETTELSS
α₂ cytoplasmic tail		KLGFFKRKYEKMTKNPDEIDETTELSS
Signal-α₂ without GFFKR	VTVLALGALAGVGVG	KL_____KYEKMTKNPDEIDETTELSS
Signal-KLGFFKR	VTVLALGALAGVGVG	KLGFFKR
Signal-scrambled α₂ tail	VTVLALGALAGVGVG	TKSKYNEGSELIKTLKMTDFFREDKEP
Signal-β₁ cytoplasmic tail	VTVLALGALAGVGVG	NPIYKSAVTTVVNPKYEGK
β₁ cytoplasmic tail		NPIYKSAVTTVVNPKYEGK

Sequences are given in the single-letter amino acid code. The cell membrane—permeable signal sequence (italicized) was derived from the hydrophobic domain of the human β₃ integrin signal sequence.²⁴ Peptides were synthesized and purified as described in “Materials and methods.” Underscore indicates deleted amino acids.

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