Table 1. Subcloning efficiency of... | American Society of Hematology

Table 1.

Subcloning efficiency of DN-hTERT (Asp712Ala, Val713Ile)-GFP, hTERT-GFP, or control GFP-transduced MOT-72 CD4⁺ T cells

	MOT-GFP			DN-hTERT-GFP			hTERT-GFP
Cells/well	Clones^*	% total	Clones^*	% total	Clones^*	% total
Experiment 1
100	48/48	100	35/40	87	48/48	100
30	40/48	83	13/40	33	41/48	85
10	14/48	29	1/48	2	8/48	17
3	9/48	19	1/48	2	1/48	2
1	5/96	5	2/96	2	8/96	8
Cloning efficiency, %	4.4	NA	1.7^†	NA	4.2	NA
Experiment 2
100	34/48	70	27/48	56	47/48	98
30	11/48	23	6/48	13	25/48	52
10	0/48	0	2/48	4	6/48	13
3	1/48	2	0/48	0	3/48	6
1	0/96	0	0/96	0	0/96	0
Cloning efficiency, %	1.2	NA	0.8^†	NA	2.7	NA

	MOT-GFP			DN-hTERT-GFP			hTERT-GFP
Cells/well	Clones^*	% total	Clones^*	% total	Clones^*	% total
Experiment 1
100	48/48	100	35/40	87	48/48	100
30	40/48	83	13/40	33	41/48	85
10	14/48	29	1/48	2	8/48	17
3	9/48	19	1/48	2	1/48	2
1	5/96	5	2/96	2	8/96	8
Cloning efficiency, %	4.4	NA	1.7^†	NA	4.2	NA
Experiment 2
100	34/48	70	27/48	56	47/48	98
30	11/48	23	6/48	13	25/48	52
10	0/48	0	2/48	4	6/48	13
3	1/48	2	0/48	0	3/48	6
1	0/96	0	0/96	0	0/96	0
Cloning efficiency, %	1.2	NA	0.8^†	NA	2.7	NA

NA indicates not applicable

Number of wells with growing cells/total number of wells seeded

All clones died shortly after cloning