Table 3. Loss of detectable telomeres in... | American Society of Hematology

Table 3.

Loss of detectable telomeres in dominant-negative hTERT-transduced CD4⁺ T cells

DNA construct used for CD4⁺ T-cell transduction	Metaphases analyzed	Chromosomes analyzed	Telomere spots^*	Mean no. of spots/chromosome	SD	Significance^† compared with GFP
GFP	16	735	2818	3.83	0.11	NA
DN-hTERT-GFP	16	733	2498	3.42	0.17	<0.001
hTERT-GFP	16	731	2861	3.91	0.09	0.038

DNA construct used for CD4⁺ T-cell transduction	Metaphases analyzed	Chromosomes analyzed	Telomere spots^*	Mean no. of spots/chromosome	SD	Significance^† compared with GFP
GFP	16	735	2818	3.83	0.11	NA
DN-hTERT-GFP	16	733	2498	3.42	0.17	<0.001
hTERT-GFP	16	731	2861	3.91	0.09	0.038

NA indicates not applicable

Total number of telomere spots measured by Q-FISH

Significance of the difference in numbers of detectable telomeres, compared with control GFP-transduced T cells, was tested in a Student t test