Primers used for real-time RT-PCR
Product . | 5′ Sequence 3′ . | Sense/antisense . | Product length, bp . | Tm, °C . |
|---|---|---|---|---|
| CSF2 | GGC CAG CCA CTA CAA GCA GCA CT | Sense | 119 | 61.4 |
| CAA AGG GGA TGA CAA GCA GAA AG | Antisense | 56.7 | ||
| EGR-1 | GAT CTC GGC TCA CTG CAA CCT CTG | Sense | 167 | 61.5 |
| CCA AGC CAG CGG GAA TG | Antisense | 58.5 | ||
| GRO-1 | CTC TAC CTG CAC ACT GTC CTA TTA | Sense | 178 | 50.1 |
| ATC CGC CAG CCT CTA TCA | Antisense | 50.3 | ||
| GRO-2 | TTT TAG GTC AAA CCC AAG TTA GTT | Sense | 150 | 50.6 |
| TTC TTG GAT TCC TCA GCC TCT ATC | Antisense | 54.6 | ||
| GRO-3 | AAG AAG CTT ATC AGC GTA TCA T | Sense | 150 | 54.7 |
| AAT AAG TAG AAC CCT CGT AAG AAA | Antisense | 55.9 | ||
| ICAM1 | TGC CCG AGC TCA AGT GTC TA | Sense | 225 | 53.3 |
| GCC TGC AGT GCC CAT TAT | Antisense | 50.7 | ||
| IL-6 | CAC CCC TGA CCC AAC CAC AAA T | Sense | 105 | 59.3 |
| TCC TTA AAG CTG CGC AGA ATG AGA | Antisense | 59.2 | ||
| IL-8 | GAC CAC ACT GCG CCA ACA CA | Sense | 117 | 57.6 |
| CAG CCC TCT TCA AAA ACT TCT CCA | Antisense | 57.8 | ||
| LAMB3 | CAG ACG CAC ACG GCT CCT AAT CCA | Sense | 225 | 63.9 |
| CGG GCA CGC GCA ATG TCC T | Antisense | 63.6 | ||
| MCP-1 | GTC TCT GCC GCC CTT CTG TG | Sense | 94 | 63.5 |
| AGG TGA CTG GGG CAT TGA TTG | Antisense | 59.8 | ||
| NFκB2 | TGC CAA ACA GAT TCC CAA AGT AGT | Sense | 155 | 55.9 |
| CAA GGA GAT GCA AAT TCA AAC ACA | Antisense | 55.8 | ||
| RPS-9 | CGC AGG CGC AGA CGG TGG AAG C | Sense | 92 | 61.1 |
| CGA AGG GTC TCC GCG GGG TCA CAT | Antisense | 60.5 | ||
| TRAF-1 | GGG GCA TAA ACT TTC CTC TTC C | Sense | 144 | 60.3 |
| TTT GGG GTT ATA CAT TGC TCA GTG | Antisense | 59.3 |
Product . | 5′ Sequence 3′ . | Sense/antisense . | Product length, bp . | Tm, °C . |
|---|---|---|---|---|
| CSF2 | GGC CAG CCA CTA CAA GCA GCA CT | Sense | 119 | 61.4 |
| CAA AGG GGA TGA CAA GCA GAA AG | Antisense | 56.7 | ||
| EGR-1 | GAT CTC GGC TCA CTG CAA CCT CTG | Sense | 167 | 61.5 |
| CCA AGC CAG CGG GAA TG | Antisense | 58.5 | ||
| GRO-1 | CTC TAC CTG CAC ACT GTC CTA TTA | Sense | 178 | 50.1 |
| ATC CGC CAG CCT CTA TCA | Antisense | 50.3 | ||
| GRO-2 | TTT TAG GTC AAA CCC AAG TTA GTT | Sense | 150 | 50.6 |
| TTC TTG GAT TCC TCA GCC TCT ATC | Antisense | 54.6 | ||
| GRO-3 | AAG AAG CTT ATC AGC GTA TCA T | Sense | 150 | 54.7 |
| AAT AAG TAG AAC CCT CGT AAG AAA | Antisense | 55.9 | ||
| ICAM1 | TGC CCG AGC TCA AGT GTC TA | Sense | 225 | 53.3 |
| GCC TGC AGT GCC CAT TAT | Antisense | 50.7 | ||
| IL-6 | CAC CCC TGA CCC AAC CAC AAA T | Sense | 105 | 59.3 |
| TCC TTA AAG CTG CGC AGA ATG AGA | Antisense | 59.2 | ||
| IL-8 | GAC CAC ACT GCG CCA ACA CA | Sense | 117 | 57.6 |
| CAG CCC TCT TCA AAA ACT TCT CCA | Antisense | 57.8 | ||
| LAMB3 | CAG ACG CAC ACG GCT CCT AAT CCA | Sense | 225 | 63.9 |
| CGG GCA CGC GCA ATG TCC T | Antisense | 63.6 | ||
| MCP-1 | GTC TCT GCC GCC CTT CTG TG | Sense | 94 | 63.5 |
| AGG TGA CTG GGG CAT TGA TTG | Antisense | 59.8 | ||
| NFκB2 | TGC CAA ACA GAT TCC CAA AGT AGT | Sense | 155 | 55.9 |
| CAA GGA GAT GCA AAT TCA AAC ACA | Antisense | 55.8 | ||
| RPS-9 | CGC AGG CGC AGA CGG TGG AAG C | Sense | 92 | 61.1 |
| CGA AGG GTC TCC GCG GGG TCA CAT | Antisense | 60.5 | ||
| TRAF-1 | GGG GCA TAA ACT TTC CTC TTC C | Sense | 144 | 60.3 |
| TTT GGG GTT ATA CAT TGC TCA GTG | Antisense | 59.3 |
CSF2 indicates colony stimulating factor 2 (granulocyte-macrophage); EGR-1, early growth response 1; GRO-1, GRO-1 oncogene; GRO-2, GRO-2 oncogene; GRO-3, GRO-3 oncogene; ICAM1, intercellular adhesion molecule-1; IL-6, interleukin 6; IL-8, interleukin 8; LAMB3, laminin beta 3; MCP-1, monocyte chemotactic protein 1; NFκB2, nuclear factor of kappa light polypeptide gene enhancer in B-cells 2; RPS-9, ribosomal protein S9; TRAF-1, TNF receptor—associated factor 1; and Tm, melting temperature.