Table 2. Effect of HbLDL pretreatment on... | American Society of Hematology

Table 2.

Effect of HbLDL pretreatment on RAEC and MPH oxidative stress resistance

	RAECs			MPHs
	nLDL	HbLDL	nLDL	HbLDL
No treatment	0.82 ± 0.23	1.1 ± 0.39*	0.49 ± 0.11	0.4 ± 0.1†
Hemin, 50 μM	0.055 ± 0.015	0.31 ± 0.22*	0.1 ± 0.03	0.15 ± 0.09†
t-BuOOH, 200 μM	0.34 ± 0.16	0.63 ± 0.31‡	0.16 ± 0.096	0.2 ± 0.14†
t-BuOOH, 500 μM	0.08 ± 0.018	0.17 ± 0.13†	0.045 ± 0.019	0.098 ± 0.07‡

	RAECs			MPHs
	nLDL	HbLDL	nLDL	HbLDL
No treatment	0.82 ± 0.23	1.1 ± 0.39*	0.49 ± 0.11	0.4 ± 0.1†
Hemin, 50 μM	0.055 ± 0.015	0.31 ± 0.22*	0.1 ± 0.03	0.15 ± 0.09†
t-BuOOH, 200 μM	0.34 ± 0.16	0.63 ± 0.31‡	0.16 ± 0.096	0.2 ± 0.14†
t-BuOOH, 500 μM	0.08 ± 0.018	0.17 ± 0.13†	0.045 ± 0.019	0.098 ± 0.07‡

Cells were pretreated overnight with 200 μg/mL nLDL or HbLDL, and then treated with hemin or t-BuOOH for 6 hours. Cell survival was determined as described in “Materials and methods.” Plating efficiencies calculated as described for LDL and HbLDL are presented.

P < .05.

†

P < .5.

‡

P < .1.

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