PCR primers for the detection of chemokine receptors and chemokines
Probe . | Product length, bp . | Sense primer sequence . | Antisense primer sequence . |
|---|---|---|---|
| Chemokine receptors | |||
| CXCR1 | 178 | TATGCCCTAGTGTTCCTGCTGA | ATCCAGCCATTCACCTTGGA |
| CXCR2 | 421 | CTTTTCTACTAGATGCCGC | CCCCAGATGCTGAGACATATGAAT |
| CXCR3 | 587 | TCCTTGAGGTGAGTGACCACCAA | GCGCTCGTCGTGGTGGGCCGACA |
| CXCR4 | 260 | AGCTGTTGGCTGAAAAGGTGGTCTATG | GCGCTTCTGGTGGCCCTTGGAGTGTG |
| CXCR5 | 316 | ATGAACTACCCGCTAACGCT | AGGGCAAGATGAAGACCAGCAGGA |
| CXCR6 | 173 | CTGGTGGTGTTTGTCTGTGG | CCATTCATGGATGCCTGCATA |
| CCR1 | 296 | TGGAAACTCCAAACACCA | CCAGTCATCCTTCAACTT |
| CCR2 | 324 | CTGTCCACATCTCGTTCTCGGTTTA | CCCAAAGACCCACTCATTTGCAGC |
| CCR3 | 366 | CTGCTACACAGGAATCATCAAAAC | GTTCTTTCCAGCTTCTCACTAGGAAG |
| CCR4 | 382 | TACTTGGTGGGCTTTTACAG | TCATCTTCACCGCCTTGTTC |
| CCR5 | 278 | AATTGCAGTAGCTCTAACAGGT | CCAGCCCACTTGAGTCCGTGTCA |
| CCR6 | 316 | GAGCCCATCAGGTGGAAGCTGCTG | GGCAGCAGTGCAGGAAAGCCAGGAC |
| CCR7 | 530 | TCCTTCTCATCAGCAAGCTGTC | GAGGCAGCCCAGGTCCTTGAA |
| CCR8 | 495 | GTGGTGTCTGGCTTTTATTACATTGG | ACATCCATCCAAGATGTGC |
| CCR9 | 103 | ATGACACCCACAGACTT | CAGTGAAGTTGAAGTTAACG |
| CCR10 | 104 | ACGGAGGCCACAGAGCAGGTTT | TGGACATCGGCCTTGTAGCA |
| CX3CR1 | 501 | TAGTGTTTGCCCTCACCAACA | ACAGCGTCTGGATGATTCTG |
| XCR1 | 528 | ATGGAGTCCTCAGGCAACCC | CACAGCCCGAAGAAAGCACCTT |
| Chemokines | |||
| CXCL9 MIG | 283 | AAGTGGTGTTCTTTTCCTCT | TTTCTTTTGGCTGACCTGTT |
| CXCL10 IP-10 | 228 | CCATGAATCAAACTGCGATT | TCAGACATCCTCTTCTCACCC |
| CXCL11 I-TAC | 230 | ATGAGTGTGAAGGGCATGG | GGATTTAGGCATCGTTGTCC |
| CXCL12-α SDF1-α | 202 | ATGAACGCCAAGGTCGTGGTCG | TGTTGTTGTTCTTCAGCCG |
| CXCL12-β SDF1-β | 220 | ATGAACGCCAAGGTCGTGGTCG | CGGGTCAATGCACACTTGTC |
Probe . | Product length, bp . | Sense primer sequence . | Antisense primer sequence . |
|---|---|---|---|
| Chemokine receptors | |||
| CXCR1 | 178 | TATGCCCTAGTGTTCCTGCTGA | ATCCAGCCATTCACCTTGGA |
| CXCR2 | 421 | CTTTTCTACTAGATGCCGC | CCCCAGATGCTGAGACATATGAAT |
| CXCR3 | 587 | TCCTTGAGGTGAGTGACCACCAA | GCGCTCGTCGTGGTGGGCCGACA |
| CXCR4 | 260 | AGCTGTTGGCTGAAAAGGTGGTCTATG | GCGCTTCTGGTGGCCCTTGGAGTGTG |
| CXCR5 | 316 | ATGAACTACCCGCTAACGCT | AGGGCAAGATGAAGACCAGCAGGA |
| CXCR6 | 173 | CTGGTGGTGTTTGTCTGTGG | CCATTCATGGATGCCTGCATA |
| CCR1 | 296 | TGGAAACTCCAAACACCA | CCAGTCATCCTTCAACTT |
| CCR2 | 324 | CTGTCCACATCTCGTTCTCGGTTTA | CCCAAAGACCCACTCATTTGCAGC |
| CCR3 | 366 | CTGCTACACAGGAATCATCAAAAC | GTTCTTTCCAGCTTCTCACTAGGAAG |
| CCR4 | 382 | TACTTGGTGGGCTTTTACAG | TCATCTTCACCGCCTTGTTC |
| CCR5 | 278 | AATTGCAGTAGCTCTAACAGGT | CCAGCCCACTTGAGTCCGTGTCA |
| CCR6 | 316 | GAGCCCATCAGGTGGAAGCTGCTG | GGCAGCAGTGCAGGAAAGCCAGGAC |
| CCR7 | 530 | TCCTTCTCATCAGCAAGCTGTC | GAGGCAGCCCAGGTCCTTGAA |
| CCR8 | 495 | GTGGTGTCTGGCTTTTATTACATTGG | ACATCCATCCAAGATGTGC |
| CCR9 | 103 | ATGACACCCACAGACTT | CAGTGAAGTTGAAGTTAACG |
| CCR10 | 104 | ACGGAGGCCACAGAGCAGGTTT | TGGACATCGGCCTTGTAGCA |
| CX3CR1 | 501 | TAGTGTTTGCCCTCACCAACA | ACAGCGTCTGGATGATTCTG |
| XCR1 | 528 | ATGGAGTCCTCAGGCAACCC | CACAGCCCGAAGAAAGCACCTT |
| Chemokines | |||
| CXCL9 MIG | 283 | AAGTGGTGTTCTTTTCCTCT | TTTCTTTTGGCTGACCTGTT |
| CXCL10 IP-10 | 228 | CCATGAATCAAACTGCGATT | TCAGACATCCTCTTCTCACCC |
| CXCL11 I-TAC | 230 | ATGAGTGTGAAGGGCATGG | GGATTTAGGCATCGTTGTCC |
| CXCL12-α SDF1-α | 202 | ATGAACGCCAAGGTCGTGGTCG | TGTTGTTGTTCTTCAGCCG |
| CXCL12-β SDF1-β | 220 | ATGAACGCCAAGGTCGTGGTCG | CGGGTCAATGCACACTTGTC |
Primers are indicated in 5′ to 3′ direction.