Semiquantative PCR analysis for mRNA expression for CXCL9, CXCL10, CXCL11, CXCL12-α, and CXCL12-β
Ligand . | Placenta tissue . | Decidua tissue . |
---|---|---|
CXCR3 | ||
CXCL10 | ++ | ++ |
CXCL9 | ++ | ++ |
CXCL11 | - | - |
CXCR4 | ||
CXCL12-α | + | + |
CXCL12-β | + | ++ |
Ligand . | Placenta tissue . | Decidua tissue . |
---|---|---|
CXCR3 | ||
CXCL10 | ++ | ++ |
CXCL9 | ++ | ++ |
CXCL11 | - | - |
CXCR4 | ||
CXCL12-α | + | + |
CXCL12-β | + | ++ |
Densitometrical analysis was performed to compare the intensities of the β-actin band with that of the various chemokines as described in “Materials and methods.” Identical results were obtained with 3 other donors. ++ indicates higher band intensity compared with β-actin band; -, no visible band; and +, lower band intensity compared with β-actin band.