Involvement of integrins αIIbβ3 and α2β1 and of bacitracin in adhesion to collagen and to receptor-specific peptides
. | Platelet adhesion, % of adhesion in the presence of ascites control . | . | . | ||
|---|---|---|---|---|---|
. | Collagen, mean ± SE . | GFOGER, mean ± SE . | CRP, mean ± SE . | ||
| Anti-αIIbβ3 | |||||
| 10E5, diluted 1:100 | 114 ± 15 (n = 3) | 113 ± 9 (n = 3) | 105 ± 7 (n = 3) | ||
| 7E3, diluted 1:100 | 115 ± 8 (n = 3) | ND | ND | ||
| GR144053F (2 μM) | 98 ± 11 (n = 3) | 109 ± 10 (n = 3) | 96 ± 2 (n = 3) | ||
| Anti-α2β1 | |||||
| 6F1 (2 μg/mL) | 12 ± 4 (n = 3) | 3 ± 3 (n = 3) | 96 ± 11 (n = 3) | ||
| Bacitracin (6 mM) | 25 ± 5 (n = 13) | 12 ± 8 (n = 4) | 77 ± 11 (n = 3) | ||
. | Platelet adhesion, % of adhesion in the presence of ascites control . | . | . | ||
|---|---|---|---|---|---|
. | Collagen, mean ± SE . | GFOGER, mean ± SE . | CRP, mean ± SE . | ||
| Anti-αIIbβ3 | |||||
| 10E5, diluted 1:100 | 114 ± 15 (n = 3) | 113 ± 9 (n = 3) | 105 ± 7 (n = 3) | ||
| 7E3, diluted 1:100 | 115 ± 8 (n = 3) | ND | ND | ||
| GR144053F (2 μM) | 98 ± 11 (n = 3) | 109 ± 10 (n = 3) | 96 ± 2 (n = 3) | ||
| Anti-α2β1 | |||||
| 6F1 (2 μg/mL) | 12 ± 4 (n = 3) | 3 ± 3 (n = 3) | 96 ± 11 (n = 3) | ||
| Bacitracin (6 mM) | 25 ± 5 (n = 13) | 12 ± 8 (n = 4) | 77 ± 11 (n = 3) | ||
Adhesion in the presence of nonimmune ascites, diluted 1:100, had no effect on adhesion to any of the substrates. ND indicates not done.