Generation of host-nonreactive anti–third party CTLs: cell recovery following different stages of the protocol
Responder/third-party stimulators 1:2 . | Culture initiation cells, × 106, day 0 . | Before CD8 selection cells, × 106, day 6 . | After CD8 selection cells, × 106, day 6 . | End of culture cells, × 106, day 18-25 . |
|---|---|---|---|---|
| Balb/c → FVB | 1875 | 183 | 36 | 492 |
| Balb/c → FVB | 2500 | 305 | 67 | 480 |
| FVB → C57BL | 1750 | 315 | 72 | 470 |
| Balb/c → FVB | 1950 | 190 | 42 | 350 |
| Balb/c → FVB | 2950 | 770 | 174 | 590 |
| Balb/c → FVB | 1115 | 135 | 23 | 230 |
| Average* | 2023 ± 579 | 316 ± 213 | 69 ± 50 | 437 ± 116 |
Responder/third-party stimulators 1:2 . | Culture initiation cells, × 106, day 0 . | Before CD8 selection cells, × 106, day 6 . | After CD8 selection cells, × 106, day 6 . | End of culture cells, × 106, day 18-25 . |
|---|---|---|---|---|
| Balb/c → FVB | 1875 | 183 | 36 | 492 |
| Balb/c → FVB | 2500 | 305 | 67 | 480 |
| FVB → C57BL | 1750 | 315 | 72 | 470 |
| Balb/c → FVB | 1950 | 190 | 42 | 350 |
| Balb/c → FVB | 2950 | 770 | 174 | 590 |
| Balb/c → FVB | 1115 | 135 | 23 | 230 |
| Average* | 2023 ± 579 | 316 ± 213 | 69 ± 50 | 437 ± 116 |
Average plus or minus standard deviation.