Flow cytometry analysis of cell populations
. | Untreated cells . | . | Camptothecin . | . | Etoposide . | . | Heat . | . | 70% ethyl alcohol . | . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
. | Jurkat . | J774 . | Jurkat . | J774 . | Jurkat . | J774 . | Jurkat . | J774 . | Jurkat . | J774 . | |||||
| Pl+ | 12.4 | 11.2 | 24.4 | 24.9 | 20.8 | 14.2 | 2.5 | 1.8 | 91.2 | 95.8 | |||||
| Pl+ Annexin V+ | 20.6 | 14.0 | 54.0 | 9.0 | 53.9 | 13.1 | 76.0 | 88.7 | 7.6 | 0.2 | |||||
| Annexin V+ | 2.8 | 11.6 | 6.2 | 21.9 | 8.1 | 12.5 | 21.4 | 9.7 | 0.7 | 0.1 | |||||
| Pl- Annexin V- | 64.2 | 63.2 | 15.4 | 44.3 | 17.3 | 60.3 | 0.2 | 0.3 | 0.6 | 3.9 | |||||
. | Untreated cells . | . | Camptothecin . | . | Etoposide . | . | Heat . | . | 70% ethyl alcohol . | . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
. | Jurkat . | J774 . | Jurkat . | J774 . | Jurkat . | J774 . | Jurkat . | J774 . | Jurkat . | J774 . | |||||
| Pl+ | 12.4 | 11.2 | 24.4 | 24.9 | 20.8 | 14.2 | 2.5 | 1.8 | 91.2 | 95.8 | |||||
| Pl+ Annexin V+ | 20.6 | 14.0 | 54.0 | 9.0 | 53.9 | 13.1 | 76.0 | 88.7 | 7.6 | 0.2 | |||||
| Annexin V+ | 2.8 | 11.6 | 6.2 | 21.9 | 8.1 | 12.5 | 21.4 | 9.7 | 0.7 | 0.1 | |||||
| Pl- Annexin V- | 64.2 | 63.2 | 15.4 | 44.3 | 17.3 | 60.3 | 0.2 | 0.3 | 0.6 | 3.9 | |||||
All data are percentages.
Cells were analyzed by flow cytometry with staining by Pl and Annexin V to assess cell populations arising from various treatments.