Table 3.

Numbers of fractionated CD4CD8 cells from FTOCs under various conditions


Condition, no. individual cultures analyzed

Total CD4-CD8- cells, × 104

Fractionated CD4-CD8- cells, × 104
CD44+CD25-
CD44+CD25+
CD44-CD25+
CD44-CD25-
Medium, n = 10   20.9 ± 7.0   6.7 ± 2.2   1.4 ± 0.5   7.8 ± 3.4   4.9 ± 1.6  
HK-1, n = 10   27.1 ± 11.1   7.5 ± 2.5   1.7 ± 0.7   10.5 ± 6.3*  7.3 ± 3.2* 
SubP, n = 10   25.1 ± 9.7   7.8 ± 3.0   2.0 ± 0.6   9.3 ± 4.6   6.0 ± 2.5* 
Antagonist, n = 9   12.1 ± 6.2   5.8 ± 2.8   1.7 ± 1.0   1.8 ± 1.9*§  2.9 ± 1.1*§ 
Antagonist + HK-1, n = 9   16.8 ± 7.6   6.8 ± 2.7   2.4 ± 1.2   3.6 ± 3.2*  4.0 ± 1.7* 
Antagonist + SubP, n = 9
 
13.4 ± 4.4
 
5.5 ± 1.7
 
1.9 ± 0.8
 
2.5 ± 1.9
 
3.4 ± 1.3
 

Condition, no. individual cultures analyzed

Total CD4-CD8- cells, × 104

Fractionated CD4-CD8- cells, × 104
CD44+CD25-
CD44+CD25+
CD44-CD25+
CD44-CD25-
Medium, n = 10   20.9 ± 7.0   6.7 ± 2.2   1.4 ± 0.5   7.8 ± 3.4   4.9 ± 1.6  
HK-1, n = 10   27.1 ± 11.1   7.5 ± 2.5   1.7 ± 0.7   10.5 ± 6.3*  7.3 ± 3.2* 
SubP, n = 10   25.1 ± 9.7   7.8 ± 3.0   2.0 ± 0.6   9.3 ± 4.6   6.0 ± 2.5* 
Antagonist, n = 9   12.1 ± 6.2   5.8 ± 2.8   1.7 ± 1.0   1.8 ± 1.9*§  2.9 ± 1.1*§ 
Antagonist + HK-1, n = 9   16.8 ± 7.6   6.8 ± 2.7   2.4 ± 1.2   3.6 ± 3.2*  4.0 ± 1.7* 
Antagonist + SubP, n = 9
 
13.4 ± 4.4
 
5.5 ± 1.7
 
1.9 ± 0.8
 
2.5 ± 1.9
 
3.4 ± 1.3
 

All data are given as the mean ± SD per lobe.

*

P < .05.

Paired, 2-tailed t tests of medium versus HK-1

Paired, 2-tailed t tests of medium versus SubP.

§

Paired, 2-tailed t tests of medium versus antagonist.

Paired, 2-tailed t tests of antagonist versus antagonist plus HK-1.

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