Cross-reactivity of anti-FVIII antibodies
. | Coagulation time, s . | . | FVIII:C, U/mL . | . | ||
|---|---|---|---|---|---|---|
. | . | Average . | . | Average . | ||
| FVIII:C (U/mL) | ||||||
| Mouse plasma - Ab | 40.5 | 40.35 | 0.81 | 0.82 | ||
| 40.2 | 0.83 | |||||
| Mouse plasma + Ab | 66.4 | 66.80 | 0.09 | 0.09 | ||
| 67.2 | 0.09 | |||||
| FV:C (U/mL) | ||||||
| Mouse plasma - Ab | 11.7 | 11.75 | 0.85 | 0.83 | ||
| 11.8 | 0.81 | |||||
| Mouse plasma + Ab | 11.5 | 11.50 | 0.90 | 0.90 | ||
| 11.5 | 0.90 | |||||
| FII:C (U/mL) | ||||||
| Mouse plasma - Ab | 19.2 | 19.25 | 0.94 | 0.93 | ||
| 19.3 | 0.92 | |||||
| Mouse plasma + Ab | 19.2 | 19.00 | 0.94 | 0.96 | ||
| 18.8 | 0.98 | |||||
| FX:C (U/mL) | ||||||
| Mouse plasma - Ab | 11.8 | 11.90 | 0.89 | 0.88 | ||
| 12.0 | 0.86 | |||||
| Mouse plasma + Ab | 11.8 | 11.65 | 0.89 | 0.93 | ||
| 11.5 | 0.93 | |||||
. | Coagulation time, s . | . | FVIII:C, U/mL . | . | ||
|---|---|---|---|---|---|---|
. | . | Average . | . | Average . | ||
| FVIII:C (U/mL) | ||||||
| Mouse plasma - Ab | 40.5 | 40.35 | 0.81 | 0.82 | ||
| 40.2 | 0.83 | |||||
| Mouse plasma + Ab | 66.4 | 66.80 | 0.09 | 0.09 | ||
| 67.2 | 0.09 | |||||
| FV:C (U/mL) | ||||||
| Mouse plasma - Ab | 11.7 | 11.75 | 0.85 | 0.83 | ||
| 11.8 | 0.81 | |||||
| Mouse plasma + Ab | 11.5 | 11.50 | 0.90 | 0.90 | ||
| 11.5 | 0.90 | |||||
| FII:C (U/mL) | ||||||
| Mouse plasma - Ab | 19.2 | 19.25 | 0.94 | 0.93 | ||
| 19.3 | 0.92 | |||||
| Mouse plasma + Ab | 19.2 | 19.00 | 0.94 | 0.96 | ||
| 18.8 | 0.98 | |||||
| FX:C (U/mL) | ||||||
| Mouse plasma - Ab | 11.8 | 11.90 | 0.89 | 0.88 | ||
| 12.0 | 0.86 | |||||
| Mouse plasma + Ab | 11.8 | 11.65 | 0.89 | 0.93 | ||
| 11.5 | 0.93 | |||||
Cross-reactivity of anti-FVIII was evaluated against FVIII, FV, FII, and FX. The cross-reactivity of the anti-FVIII antibodies to other relevant coagulation proteins (FV, FX, and FII) was tested in a standard assay and revealed no effects on FV, FII, and FX, while FVIII activity was efficiently neutralized.