The functional activity and tetramer staining of HA-2/HLA-A2 tetramer-positive T-cell clones isolated from an antileukemic response
. | . | Lytic activity, 4 h, % lysis . | . | Cytokine production, pg/mL . | . | . | . | . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| HA-2 clone no. . | HLA-A2/HA-2 MFI . | EBV donor . | EBV patient . | IFN-γ . | TNF-α . | IL-10 . | IL-5 . | IL-4 . | |||||
| HA-2.01 | 206 | 0 | 69 | >5000 | 6 | 585 | 93 | 5 | |||||
| HA-2.02 | 735 | 4 | 64 | >5000 | 0 | 22 | 38 | 5 | |||||
| HA-2.03 | 553 | 6 | 61 | 3619 | 10 | 11 | 653 | 7 | |||||
| HA-2.04 | 306 | 0 | 72 | 3944 | 6 | 11 | 104 | 12 | |||||
| HA-2.05 | 466 | 0 | 79 | >5000 | 10 | 98 | 268 | 34 | |||||
| HA-2.06 | 630 | 0 | 53 | 1375 | 6 | 8 | 248 | 6 | |||||
| HA-2.07 | 520 | 0 | 60 | 4100 | 4 | 205 | 42 | 0 | |||||
| HA-2.08 | 256 | 2 | 64 | >5000 | 25 | 20 | 2433 | 72 | |||||
| HA-2.09 | 638 | 4 | 56 | 4709 | 8 | 9 | 638 | 9 | |||||
| HA-2.10 | 545 | 0 | 66 | 3914 | 86 | 13 | 19 | 5 | |||||
| HA-2.11 | 514 | 4 | 61 | 3319 | 3 | 6 | 53 | 4 | |||||
| HA-2.12 | 601 | 4 | 67 | 1900 | 0 | 6 | 13 | 4 | |||||
| HA-2.13 | 640 | 9 | 64 | 1477 | 10 | 5 | 287 | 39 | |||||
| HA-2.15 | 666 | 0 | 61 | 1476 | 9 | 16 | 138 | 7 | |||||
| HA-2.17 | 208 | 4 | 76 | 3412 | 21 | 62 | 2151 | 78 | |||||
| HA-2.19 | 689 | 0 | 71 | >5000 | 15 | 6 | 99 | 44 | |||||
| HA-2.20 | 468 | 2 | 61 | 3293 | 11 | 13 | 1414 | 13 | |||||
| HA-2.21 | 496 | 1 | 59 | 2211 | 6 | 4 | 65 | 2 | |||||
| HA-2.22 | 533 | 0 | 69 | 803 | 7 | 0 | 131 | 3 | |||||
| HA-2.24 | 777 | 0 | 63 | 1188 | 4 | 2 | 13 | 4 | |||||
| HA-2.25 | 327 | 1 | 71 | 3319 | 30 | 197 | 702 | 31 | |||||
| HA-2.27 | 456 | 3 | 70 | 2211 | 25 | 3 | 3761 | 269 | |||||
| HA-2.28 | 646 | 0 | 71 | 2683 | 15 | 1 | 80 | 5 | |||||
| HA-2.30 | 671 | 0 | 14 | 207 | 0 | 2 | 3 | 2 | |||||
| HA-2.31 | 631 | 2 | 56 | 3372 | 81 | 30 | 1262 | 93 | |||||
| HA-2.32 | 362 | 0 | 54 | 3139 | 12 | 15 | 575 | 42 | |||||
| HA-2.33 | 468 | 0 | 48 | 1250 | 13 | 4 | 15 | 4 | |||||
| HA-2.34 | 341 | 7 | 61 | 4818 | 17 | 16 | 85 | 9 | |||||
| HA-2.35 | 24 | 1 | 5 | 263 | 5 | 14 | 5 | 4 | |||||
| HA-2.36 | 555 | 3 | 61 | 2184 | 13 | 0 | 20 | 5 | |||||
| HA-2.37 | 696 | 0 | 75 | 1399 | 18 | 0 | 97 | 30 | |||||
| HA-2.38 | 510 | 0 | 81 | 3372 | 15 | 0 | 206 | 7 | |||||
| HA-2.41 | 228 | 5 | 83 | 2248 | 10 | 17 | 203 | 11 | |||||
| HA-2.43 | 590 | 0 | 69 | 4532 | 7 | 28 | 83 | 4 | |||||
| HA-2.46 | 894 | 0 | 78 | 2968 | 12 | 5 | 2 | 6 | |||||
| HA-2.47 | 450 | 0 | 77 | 1982 | 20 | 102 | 139 | 5 | |||||
| HA-2.48 | 488 | 0 | 73 | 4463 | 5 | 6 | 3 | 4 | |||||
| HA-2.52 | 551 | 1 | 68 | >5000 | 33 | 16 | 167 | 33 | |||||
| HA-2.53 | 580 | 0 | 68 | >5000 | 81 | 11 | 10 | 4 | |||||
| HA-2.54 | 568 | 0 | 67 | 4709 | 49 | 6 | 3 | 0 | |||||
| HA-2.55 | 769 | 5 | 67 | >5000 | 28 | 15 | 22 | 9 | |||||
| HA-2.58 | 364 | 0 | 55 | 1211 | 7 | 7 | 68 | 6 | |||||
| HA-2.59 | 427 | 0 | 60 | 890 | 23 | 10 | 783 | 67 | |||||
| HA-2.60 | 380 | 0 | 74 | 4745 | 4 | 3 | 17 | 7 | |||||
| HA-2.61 | 300 | 0 | 73 | 2324 | 32 | 25 | 63 | 13 | |||||
| HA-2.62 | 564 | 0 | 85 | 3648 | 42 | 12 | 9 | 14 | |||||
| HA-2.65 | 379 | 0 | 60 | 2608 | 3 | 16 | 865 | 62 | |||||
| HA-2.67 | 252 | 5 | 60 | >5000 | 9 | 2 | 16 | 5 | |||||
| HA-2.68 | 562 | 0 | 57 | 2462 | 13 | 8 | 226 | 49 | |||||
| HA-2.72 | 508 | 0 | 69 | 2129 | 3 | 3 | 1234 | 3 | |||||
| HA-2.73 | 394 | 0 | 70 | 3535 | 6 | 5 | 50 | 9 | |||||
| HA-2.74 | 615 | 0 | 75 | 2661 | 4 | 5 | 123 | 2 | |||||
| HA-2.75 | 420 | 3 | 81 | 2344 | 15 | 6 | 72 | 6 | |||||
. | . | Lytic activity, 4 h, % lysis . | . | Cytokine production, pg/mL . | . | . | . | . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| HA-2 clone no. . | HLA-A2/HA-2 MFI . | EBV donor . | EBV patient . | IFN-γ . | TNF-α . | IL-10 . | IL-5 . | IL-4 . | |||||
| HA-2.01 | 206 | 0 | 69 | >5000 | 6 | 585 | 93 | 5 | |||||
| HA-2.02 | 735 | 4 | 64 | >5000 | 0 | 22 | 38 | 5 | |||||
| HA-2.03 | 553 | 6 | 61 | 3619 | 10 | 11 | 653 | 7 | |||||
| HA-2.04 | 306 | 0 | 72 | 3944 | 6 | 11 | 104 | 12 | |||||
| HA-2.05 | 466 | 0 | 79 | >5000 | 10 | 98 | 268 | 34 | |||||
| HA-2.06 | 630 | 0 | 53 | 1375 | 6 | 8 | 248 | 6 | |||||
| HA-2.07 | 520 | 0 | 60 | 4100 | 4 | 205 | 42 | 0 | |||||
| HA-2.08 | 256 | 2 | 64 | >5000 | 25 | 20 | 2433 | 72 | |||||
| HA-2.09 | 638 | 4 | 56 | 4709 | 8 | 9 | 638 | 9 | |||||
| HA-2.10 | 545 | 0 | 66 | 3914 | 86 | 13 | 19 | 5 | |||||
| HA-2.11 | 514 | 4 | 61 | 3319 | 3 | 6 | 53 | 4 | |||||
| HA-2.12 | 601 | 4 | 67 | 1900 | 0 | 6 | 13 | 4 | |||||
| HA-2.13 | 640 | 9 | 64 | 1477 | 10 | 5 | 287 | 39 | |||||
| HA-2.15 | 666 | 0 | 61 | 1476 | 9 | 16 | 138 | 7 | |||||
| HA-2.17 | 208 | 4 | 76 | 3412 | 21 | 62 | 2151 | 78 | |||||
| HA-2.19 | 689 | 0 | 71 | >5000 | 15 | 6 | 99 | 44 | |||||
| HA-2.20 | 468 | 2 | 61 | 3293 | 11 | 13 | 1414 | 13 | |||||
| HA-2.21 | 496 | 1 | 59 | 2211 | 6 | 4 | 65 | 2 | |||||
| HA-2.22 | 533 | 0 | 69 | 803 | 7 | 0 | 131 | 3 | |||||
| HA-2.24 | 777 | 0 | 63 | 1188 | 4 | 2 | 13 | 4 | |||||
| HA-2.25 | 327 | 1 | 71 | 3319 | 30 | 197 | 702 | 31 | |||||
| HA-2.27 | 456 | 3 | 70 | 2211 | 25 | 3 | 3761 | 269 | |||||
| HA-2.28 | 646 | 0 | 71 | 2683 | 15 | 1 | 80 | 5 | |||||
| HA-2.30 | 671 | 0 | 14 | 207 | 0 | 2 | 3 | 2 | |||||
| HA-2.31 | 631 | 2 | 56 | 3372 | 81 | 30 | 1262 | 93 | |||||
| HA-2.32 | 362 | 0 | 54 | 3139 | 12 | 15 | 575 | 42 | |||||
| HA-2.33 | 468 | 0 | 48 | 1250 | 13 | 4 | 15 | 4 | |||||
| HA-2.34 | 341 | 7 | 61 | 4818 | 17 | 16 | 85 | 9 | |||||
| HA-2.35 | 24 | 1 | 5 | 263 | 5 | 14 | 5 | 4 | |||||
| HA-2.36 | 555 | 3 | 61 | 2184 | 13 | 0 | 20 | 5 | |||||
| HA-2.37 | 696 | 0 | 75 | 1399 | 18 | 0 | 97 | 30 | |||||
| HA-2.38 | 510 | 0 | 81 | 3372 | 15 | 0 | 206 | 7 | |||||
| HA-2.41 | 228 | 5 | 83 | 2248 | 10 | 17 | 203 | 11 | |||||
| HA-2.43 | 590 | 0 | 69 | 4532 | 7 | 28 | 83 | 4 | |||||
| HA-2.46 | 894 | 0 | 78 | 2968 | 12 | 5 | 2 | 6 | |||||
| HA-2.47 | 450 | 0 | 77 | 1982 | 20 | 102 | 139 | 5 | |||||
| HA-2.48 | 488 | 0 | 73 | 4463 | 5 | 6 | 3 | 4 | |||||
| HA-2.52 | 551 | 1 | 68 | >5000 | 33 | 16 | 167 | 33 | |||||
| HA-2.53 | 580 | 0 | 68 | >5000 | 81 | 11 | 10 | 4 | |||||
| HA-2.54 | 568 | 0 | 67 | 4709 | 49 | 6 | 3 | 0 | |||||
| HA-2.55 | 769 | 5 | 67 | >5000 | 28 | 15 | 22 | 9 | |||||
| HA-2.58 | 364 | 0 | 55 | 1211 | 7 | 7 | 68 | 6 | |||||
| HA-2.59 | 427 | 0 | 60 | 890 | 23 | 10 | 783 | 67 | |||||
| HA-2.60 | 380 | 0 | 74 | 4745 | 4 | 3 | 17 | 7 | |||||
| HA-2.61 | 300 | 0 | 73 | 2324 | 32 | 25 | 63 | 13 | |||||
| HA-2.62 | 564 | 0 | 85 | 3648 | 42 | 12 | 9 | 14 | |||||
| HA-2.65 | 379 | 0 | 60 | 2608 | 3 | 16 | 865 | 62 | |||||
| HA-2.67 | 252 | 5 | 60 | >5000 | 9 | 2 | 16 | 5 | |||||
| HA-2.68 | 562 | 0 | 57 | 2462 | 13 | 8 | 226 | 49 | |||||
| HA-2.72 | 508 | 0 | 69 | 2129 | 3 | 3 | 1234 | 3 | |||||
| HA-2.73 | 394 | 0 | 70 | 3535 | 6 | 5 | 50 | 9 | |||||
| HA-2.74 | 615 | 0 | 75 | 2661 | 4 | 5 | 123 | 2 | |||||
| HA-2.75 | 420 | 3 | 81 | 2344 | 15 | 6 | 72 | 6 | |||||
The HA-2/HLA-A2 tetramer staining is shown as MFI, the cytokine production upon specific stimulation with HLA-A2-positive HA-2-positive stimulator cells (EBV-patient) is shown. The background cytokine production by the different HA-2-specific T-cell clones upon stimulation with HLA-A2-positive HA-2-negative stimulator cells (EBV-donor) is not shown. The background production of IFN-γ < 200 pg/mL, TNF-α < 5 pg/mL, IL-10 < 20 pg/mL, IL-5 < 100 pg/mL, IL-4 < 20 pg/mL.