Adoptive transfer of Aspergillus-specific CD4+ T cells in mice with IA undergoing HSCT
. | . | A fumigatus infection . | . | |
|---|---|---|---|---|
| Type of cells . | No. of cells . | MST, d . | D/T . | |
| — | — | 5.5 | 8/8 | |
| Unstimulated | 5 × 105 | 6 | 8/8 | |
| Aspergillus specific | 5 × 105 | 18* | 8/8 | |
| Aspergillus specific | 5 × 106 | 16* | 8/8 | |
. | . | A fumigatus infection . | . | |
|---|---|---|---|---|
| Type of cells . | No. of cells . | MST, d . | D/T . | |
| — | — | 5.5 | 8/8 | |
| Unstimulated | 5 × 105 | 6 | 8/8 | |
| Aspergillus specific | 5 × 105 | 18* | 8/8 | |
| Aspergillus specific | 5 × 106 | 16* | 8/8 | |
Splenic CD4+ T cells were from BALB/c mice either untreated or treated with A fumigatus CCFAs before intranasal infection with Aspergillus conidia (“Materials and methods”). Two weeks later, cells were restimulated in vitro with CCFAs and purified spleen DCs for 3 days before adoptive transfer (intravenously) into mice receiving HSC transplants, a day after the infection. Mice received 2 × 108Aspergillus conidia intratracheally, a week after transplantation. MST indicates median survival time (days); D/T, dead animals over total injected. — indicates no cells transferred.
P<.01 (treated versus untreated).