Effect of ifosfamide and BSO on DC viability
. | Cell labeling, % . | . | . | . | |||
|---|---|---|---|---|---|---|---|
. | Annexin V-PI− . | Annexin V+ . | Annexin V+PI+ . | PI+ . | |||
| Control | 81.8 ± 5.7 | 8.5 ± 2 | 8 ± 3.8 | 1.5 ± 0.9 | |||
| 4-OH-IF | 80 ± 6.4 | 9.6 ± 1.8 | 9 ± 4.5 | 1.2 ± 0.6 | |||
| BSO | 82.3 ± 5.3 | 8.47 ± 1.6 | 7.5 ± 3.5 | 1.7 ± 0.5 | |||
| Control* | 81 ± 16 | 12 ± 10 | 10 ± 9 | 7 ± 6 | |||
| 4-OH-IF* | 84 ± 13 | 9 ± 8 | 10 ± 8 | 6 ± 4 | |||
. | Cell labeling, % . | . | . | . | |||
|---|---|---|---|---|---|---|---|
. | Annexin V-PI− . | Annexin V+ . | Annexin V+PI+ . | PI+ . | |||
| Control | 81.8 ± 5.7 | 8.5 ± 2 | 8 ± 3.8 | 1.5 ± 0.9 | |||
| 4-OH-IF | 80 ± 6.4 | 9.6 ± 1.8 | 9 ± 4.5 | 1.2 ± 0.6 | |||
| BSO | 82.3 ± 5.3 | 8.47 ± 1.6 | 7.5 ± 3.5 | 1.7 ± 0.5 | |||
| Control* | 81 ± 16 | 12 ± 10 | 10 ± 9 | 7 ± 6 | |||
| 4-OH-IF* | 84 ± 13 | 9 ± 8 | 10 ± 8 | 6 ± 4 | |||
Immature DCs derived from monocytes were treated with 4-OH-IF (100 μM) on day 8 for 90 minutes or with BSO (5 mM) for 24 hours on day 7 and were tested for viability. DCs were also incubated with 4-OH-IF for 90 minutes and tested for viability 48 hours later.
Cells were then washed and incubated with FITC-conjugated Annexin V for 10 minutes at room temperature. PI (1 μg/mL) was added before flow cytometry analysis. Annexin V+ staining indicates cells in early apoptosis, Annexin V+PI+ staining indicates late apoptosis, and PI+ staining alone indicates necrotic cells. Data represent the mean ± SD of percentage positive cells in each quadrant of a dot plot analysis from 4 different donors.