Effect of BSO and 4-OH-IF on MHC and costimulatory molecule expression on DCs
Surface marker . | Control, % (MFI) . | BSO, % (MFI) . | 4-OH-IF, % (MFI) . |
|---|---|---|---|
| HLA-DR | 99 ± 1 (349 ± 137) | 100 (395 ± 206) | 100 (342 ± 111) |
| MHC class I | 99 ± 1 (216 ± 104) | 99 ± 1 (197 ± 88) | 100 (181 ± 59) |
| CD86 | 25 ± 13 (76 ± 19) | 26 ± 18 (65 ± 16) | 23 ± 14 (66 ± 22) |
| CD40 | 98 ± 1 (66 ± 30) | 97 ± 1 (52 ± 13) | 79 ± 5 (31 ± 1) |
| CD83 | 22 ± 11 (36 ± 4) | 22 ± 14 (34 ± 3) | 19 ± 10 (34 ± 2) |
Surface marker . | Control, % (MFI) . | BSO, % (MFI) . | 4-OH-IF, % (MFI) . |
|---|---|---|---|
| HLA-DR | 99 ± 1 (349 ± 137) | 100 (395 ± 206) | 100 (342 ± 111) |
| MHC class I | 99 ± 1 (216 ± 104) | 99 ± 1 (197 ± 88) | 100 (181 ± 59) |
| CD86 | 25 ± 13 (76 ± 19) | 26 ± 18 (65 ± 16) | 23 ± 14 (66 ± 22) |
| CD40 | 98 ± 1 (66 ± 30) | 97 ± 1 (52 ± 13) | 79 ± 5 (31 ± 1) |
| CD83 | 22 ± 11 (36 ± 4) | 22 ± 14 (34 ± 3) | 19 ± 10 (34 ± 2) |
DCs were cultured for 24 hours in the presence of BSO (0.5 mM) on day 7 or with 4-OH-IF (100 μM) for 90 minutes on day 8. Cells were then washed and stained with antibodies to the above molecules. Surface marker expression was analyzed by FACS analysis. Results are expressed as the percentage positive cells and the mean fluorescence intensity (MFI) and represent the mean ± SD of 3 experiments from 3 donors.