Parameters of thrombin- and batroxobin-catalyzed polymerization of control and BβArg14His fibrinogens
. | Control . | BβArg14His . | P . |
|---|---|---|---|
| Thrombin | |||
| Lag time, sec | 90 ± 23 | 230 ± 28 | < .0001 |
| Rateagg, × 10−4 U/sec | 7.0 ± 0.2 | 2.3 ± 0.2 | .0004 |
| Final OD | 0.36 ± 0.08 | 0.28 ± 0.05 | .07 |
| Batroxobin | |||
| Lag time, sec | 320 ± 46 | 710 ± 88 | < .0001 |
| Rateagg, × 10−4U/sec | 2.8 ± 0.2 | 1.5 ± 0.1 | < .0001 |
| Final OD | 0.30 ± 0.03 | 0.16 ± 0.01 | < .0001 |
| desA fibrin monomers | |||
| Lag time, sec | 45 ± 20 | None observed | NA |
| Rateagg, × 10−4U/sec | 154 ± 44 | 39 ± 11 | .002 |
| Final OD | 0.051 ± 0.01 | 0.041 ± 0.01 | .22 |
. | Control . | BβArg14His . | P . |
|---|---|---|---|
| Thrombin | |||
| Lag time, sec | 90 ± 23 | 230 ± 28 | < .0001 |
| Rateagg, × 10−4 U/sec | 7.0 ± 0.2 | 2.3 ± 0.2 | .0004 |
| Final OD | 0.36 ± 0.08 | 0.28 ± 0.05 | .07 |
| Batroxobin | |||
| Lag time, sec | 320 ± 46 | 710 ± 88 | < .0001 |
| Rateagg, × 10−4U/sec | 2.8 ± 0.2 | 1.5 ± 0.1 | < .0001 |
| Final OD | 0.30 ± 0.03 | 0.16 ± 0.01 | < .0001 |
| desA fibrin monomers | |||
| Lag time, sec | 45 ± 20 | None observed | NA |
| Rateagg, × 10−4U/sec | 154 ± 44 | 39 ± 11 | .002 |
| Final OD | 0.051 ± 0.01 | 0.041 ± 0.01 | .22 |
OD indicates optical density at 350 nm; NA, not applicable.