Mobilization of rhesus peripheral blood pre-DC subsets in response to Flt3L
. | . | CD11c+ pre-DCs . | . | CD123++ pre-DCs . | . | ||
|---|---|---|---|---|---|---|---|
| Time of sample . | Lin− MHC 2+, %PBMCs . | MHC 2+ PBMCs, % Lin− . | Absolute no. (×103/mL) . | MHC 2+ PBMCs, % Lin− . | Absolute no. (×103/mL) . | ||
| Before Flt3L (n = 3) | 0.82 ± 0.14 | 38.0 ± 1.1 | 18.0 ± 2.6 | 3.3 ± 1.1 | 1.3 ± 0.2 | ||
| After Flt3L (n = 3) | 10.4 ± 5.3* | 15.2 ± 13.0 | 127.3 ± 61.0 | 1.3 ± 0.9 | 6.7 ± 0.8 | ||
. | . | CD11c+ pre-DCs . | . | CD123++ pre-DCs . | . | ||
|---|---|---|---|---|---|---|---|
| Time of sample . | Lin− MHC 2+, %PBMCs . | MHC 2+ PBMCs, % Lin− . | Absolute no. (×103/mL) . | MHC 2+ PBMCs, % Lin− . | Absolute no. (×103/mL) . | ||
| Before Flt3L (n = 3) | 0.82 ± 0.14 | 38.0 ± 1.1 | 18.0 ± 2.6 | 3.3 ± 1.1 | 1.3 ± 0.2 | ||
| After Flt3L (n = 3) | 10.4 ± 5.3* | 15.2 ± 13.0 | 127.3 ± 61.0 | 1.3 ± 0.9 | 6.7 ± 0.8 | ||
Mobilization of rhesus CD11c+ pre-DC and CD123++ pre-DC subsets into the peripheral circulation after 10 days of rhFlt3L administration. Data show the percentage of Lin− MHC 2+ PBMCs before and after Flt3L. Absolute cell numbers were calculated by multiplying the percentage of each DC subset within the Lin− MHC 2+ fraction by the percentage of Lin− MHC PBMCs, multiplied by the peripheral mononuclear cell count.
P = .005 compared with pre-Flt3L (Mann-Whitney U test).