Flow cytometry to detect secretion in response to platelet activation
. | % gated in positive peak . | . | . | ||
|---|---|---|---|---|---|
| . | Fibrinogen . | . | P-selectin IIa, 0.2 U/mL . | ||
| RO318220, μM . | Unactivated . | IIa, 0.2 U/mL . | . | ||
| 0 | 0.2 | 30.0 | 100 | ||
| 4.4 | 0.4 | 24.2 | — | ||
| 16.7 | 0.3 | 2.4 | 0 | ||
| 33.3 | 0.3 | 1.0 | — | ||
. | % gated in positive peak . | . | . | ||
|---|---|---|---|---|---|
| . | Fibrinogen . | . | P-selectin IIa, 0.2 U/mL . | ||
| RO318220, μM . | Unactivated . | IIa, 0.2 U/mL . | . | ||
| 0 | 0.2 | 30.0 | 100 | ||
| 4.4 | 0.4 | 24.2 | — | ||
| 16.7 | 0.3 | 2.4 | 0 | ||
| 33.3 | 0.3 | 1.0 | — | ||
Gel-filtered platelets treated for 15 minutes with DMSO or RO318220 were incubated in either FITC-labeled antifibrinogen or FITC-labeled anti-P-selectin with or without prior activation by thrombin. The platelet population was defined with FITC-anti-GP1b and unlabeled platelets were used to define the negative population. Unactivated platelets showed no positive fluorescence when incubated with FITC-labeled anti-P-selectin.
—indicates not assayed.