Effect of RO318220 on aminophospholipid exposure
. | Annexin V binding . | . | . | |
|---|---|---|---|---|
. | % platelets positive IIa, 0.2 U/mL (± SE) . | Relative fluorescence IIa, 0.2 U/mL (± SE) . | Platelets as % positive events . | |
| RO318220, μM | ||||
| 0 | 4 (1) | 2100 (550) | 88 | |
| 10 | 40 | 1113 | 97 | |
| 20 | 41 (21) | 1013 (750) | 96 | |
| 50 | 98 | 838 | — | |
| 100 | 86 (21) | 144 (90) | 100 | |
. | Annexin V binding . | . | . | |
|---|---|---|---|---|
. | % platelets positive IIa, 0.2 U/mL (± SE) . | Relative fluorescence IIa, 0.2 U/mL (± SE) . | Platelets as % positive events . | |
| RO318220, μM | ||||
| 0 | 4 (1) | 2100 (550) | 88 | |
| 10 | 40 | 1113 | 97 | |
| 20 | 41 (21) | 1013 (750) | 96 | |
| 50 | 98 | 838 | — | |
| 100 | 86 (21) | 144 (90) | 100 | |
Gel-filtered, aspirin-treated platelets treated with DMSO or RO318220 were activated with thrombin (0.2 U/mL) in the presence of FITC-labeled annexin V. The platelet population was defined by positive FITC-anti-GP1bα fluorescence. Compensation was set to minimize RO318220 fluorescence spillover into either FL-2 or FL-1. The negative gates were set with an EDTA control. Positive events were analyzed within and outside the platelet population. Results were analyzed from 5 experiments.
—indicates not assayed.