GATA-1 primers used in this study
Genomic DNA primer . | Primer sequence . | nt* . |
|---|---|---|
| Exon 2 | ||
| GATA-1 S2 | 5′ TGA GGT GAT GGA GTG GGA GGA GGG 3′ | 322 |
| GATA-1 AS2-2 | 5′ CAG CGT CCC TGT AGT AGG CCA GTG 3′ | 322 |
| GATA-1 S | 5′ GAT CCT GCT CTG GTG TCC TCC 3′ | 299 |
| GATA-1 AS2 | 5′ GGT CGG CAC ATC CAT TTG AGA AGC 3′ | 299 |
| Exon 3 | ||
| GATA-1 S3 | 5′ CCA TGT TGG GGG TGC TGG GAA CCA 3′ | 270 |
| GATA-1 AS1 | 5′ GCT GGT GCT GCC TTT TCC ATC CAG 3′ | 270 |
| GATA-1 CS S1 | 5′ GGA TCC CAG GGG GCT CAC CAT A 3′ | 440 |
| GATA-1 AS3 | 5′ GTG GGG TGG AGA GGA GAA GAG GGA 3′ | 440 |
| Exon 4 | ||
| GATA-1 S4 | 5′ AAA AAG GAC AGG GAA GTT GAG GTG 3′ | 418 |
| GATA-1 AS4 | 5′ TGT GTA GGA TGA AGG CAA GGG TTT 3′ | 418 |
| Exon 5 | ||
| GATA-1 S5 | 5′ CCT CAC TTC TTG GGT CCT CCT GA 3′ | 310 |
| GATA-1 AS5 | 5′ AGA GAG ACC TAC AAC AGG AAC AGA 3′ | 310 |
| Exon 6 | ||
| GATA-1 S6 | 5′ GCA CCC AAA AAT TAT CTT ACC CTG 3′ | 295 |
| GATA-1 CS AS | 5′ TGA AGC CAC CTC CCC ACA ATT CCC 3′ | 295 |
| GATA-1 CS S4 | 5′ CTG GAA AAG GGA AAA AGA AAC GGG 3′ | 432 |
| GATA-1 3′ AS | 5′ AAG GTT CAA GCC AGG GGG TGC CTG 3′ | 432 |
| cDNA | ||
| GATA-1 S1 | 5′ CAC ACT GAG CTT GCC ACA TCC CCA 3′ | 325 |
| GATA-1 AS | 5′ ACA GTT GAG CAA TGG GTA CAC C 3′ | 325 |
| GATA-1 S | 5′ GAT CCT GCT CTG GTG TCC TCC 3′ | 336 |
| GATA-1 AS1 | 5′ GCT GGT GCT GCC TTT TCC ATC CAG 3′ | 336 |
| GATA-1 CS S1 | 5′ GGA TCC CAG GGG GCT CAC CAT A 3′ | 412 |
| GATA-1 CS AS1 | 5′ TAG TGG CCT GTC CTG TCC CTC CGC 3′ | 412 |
| GATA-1 CS S2 | 5′ CAG TAC CTT CTT TTC TCC CAC CG 3′ | 367 |
| GF-1 | 5′ CAC CTG GTG TAG CTT GTA GTA GAG 3′ | 367 |
| GATA-1 CS S3 | 5′ GAT TGT CAG TAA ACG GGC AGG TA 3′ | 310 |
| GATA-1 CS AS2 | 5′ TGA AGC CAC CTC CCC ACA ATT CCC 3′ | 310 |
| GATA-1 CS S4 | 5′ CTG GAA AAG GGA AAA AGA AAC GGG 3′ | 423 |
| GATA-1 3′ AS | 5′ AAG GTT CAA GCC AGG GGG TGC CTG 3′ | 423 |
Genomic DNA primer . | Primer sequence . | nt* . |
|---|---|---|
| Exon 2 | ||
| GATA-1 S2 | 5′ TGA GGT GAT GGA GTG GGA GGA GGG 3′ | 322 |
| GATA-1 AS2-2 | 5′ CAG CGT CCC TGT AGT AGG CCA GTG 3′ | 322 |
| GATA-1 S | 5′ GAT CCT GCT CTG GTG TCC TCC 3′ | 299 |
| GATA-1 AS2 | 5′ GGT CGG CAC ATC CAT TTG AGA AGC 3′ | 299 |
| Exon 3 | ||
| GATA-1 S3 | 5′ CCA TGT TGG GGG TGC TGG GAA CCA 3′ | 270 |
| GATA-1 AS1 | 5′ GCT GGT GCT GCC TTT TCC ATC CAG 3′ | 270 |
| GATA-1 CS S1 | 5′ GGA TCC CAG GGG GCT CAC CAT A 3′ | 440 |
| GATA-1 AS3 | 5′ GTG GGG TGG AGA GGA GAA GAG GGA 3′ | 440 |
| Exon 4 | ||
| GATA-1 S4 | 5′ AAA AAG GAC AGG GAA GTT GAG GTG 3′ | 418 |
| GATA-1 AS4 | 5′ TGT GTA GGA TGA AGG CAA GGG TTT 3′ | 418 |
| Exon 5 | ||
| GATA-1 S5 | 5′ CCT CAC TTC TTG GGT CCT CCT GA 3′ | 310 |
| GATA-1 AS5 | 5′ AGA GAG ACC TAC AAC AGG AAC AGA 3′ | 310 |
| Exon 6 | ||
| GATA-1 S6 | 5′ GCA CCC AAA AAT TAT CTT ACC CTG 3′ | 295 |
| GATA-1 CS AS | 5′ TGA AGC CAC CTC CCC ACA ATT CCC 3′ | 295 |
| GATA-1 CS S4 | 5′ CTG GAA AAG GGA AAA AGA AAC GGG 3′ | 432 |
| GATA-1 3′ AS | 5′ AAG GTT CAA GCC AGG GGG TGC CTG 3′ | 432 |
| cDNA | ||
| GATA-1 S1 | 5′ CAC ACT GAG CTT GCC ACA TCC CCA 3′ | 325 |
| GATA-1 AS | 5′ ACA GTT GAG CAA TGG GTA CAC C 3′ | 325 |
| GATA-1 S | 5′ GAT CCT GCT CTG GTG TCC TCC 3′ | 336 |
| GATA-1 AS1 | 5′ GCT GGT GCT GCC TTT TCC ATC CAG 3′ | 336 |
| GATA-1 CS S1 | 5′ GGA TCC CAG GGG GCT CAC CAT A 3′ | 412 |
| GATA-1 CS AS1 | 5′ TAG TGG CCT GTC CTG TCC CTC CGC 3′ | 412 |
| GATA-1 CS S2 | 5′ CAG TAC CTT CTT TTC TCC CAC CG 3′ | 367 |
| GF-1 | 5′ CAC CTG GTG TAG CTT GTA GTA GAG 3′ | 367 |
| GATA-1 CS S3 | 5′ GAT TGT CAG TAA ACG GGC AGG TA 3′ | 310 |
| GATA-1 CS AS2 | 5′ TGA AGC CAC CTC CCC ACA ATT CCC 3′ | 310 |
| GATA-1 CS S4 | 5′ CTG GAA AAG GGA AAA AGA AAC GGG 3′ | 423 |
| GATA-1 3′ AS | 5′ AAG GTT CAA GCC AGG GGG TGC CTG 3′ | 423 |
Number of nucleotides (nt) in the amplified fragments.