Sequence and accession numbers of primers and hybridization probes
Target . | Primer and hyridization probes 5′→3′ . | GenBank accession no. . | Position . |
|---|---|---|---|
| AML1-ETO | |||
| Primer forward | GAGGGAAAAGCTTCACTCTG | HS-AML1M | 1993-2012 |
| Primer reverse | TCGGGTGAAATGTCATTGCC | HSETO | 340-321 |
| Sensor | CCCTCACCACCCAATGGCTTCAGC-FL | HSETO | 183-206 |
| Anchor | LCRed640-TGGGCCTTCCTCTTCTTCCTCCTCC-P | HSETO | 209-234 |
| CBFB-MYH11 | |||
| Primer forward | GCAGGCAAGGTATATTTGAAGG | HUMCBFB | 253-274 |
| Primer reverse type A | CTCTTCTCCTCATTCTGCTC | HSSMMHC | 1500-1481 |
| Primer reverse type B | TTGAGCATTTTGTTGGTCCG | HSSMMHC | 1257-1238 |
| Primer reverse type C | CTTCCAGAGCTTCCACGGT | HSSMMHC | 902-884 |
| Primer reverse type D | TCCCTGTGACGCTCTCAACT | HSSMMHC | 611-592 |
| Primer reverse type E | GGCCAGGTCTGCGTTCTCT | HSSMMHC | 439-421 |
| Primer reverse type F | TCCTCTTCTCCTCATTCTGCTC | HSSMMHC | 1502-1481 |
| Sensor | GGCTCGCTCCTCATCAAACTCCA-FL | HUMCBFB | 375-353 |
| Anchor | LCRed640-ACAGCCCATACCATCCAGTCTTTGG-P | HUMCBFB | 351-327 |
| PML-RARA | |||
| Primer bcr1 (L-type) forward | GTCTTCCTGCCCAACAGCAACC | HUMPML3 | 1623-1644 |
| Primer bcr3 (S-type) forward | AGCTCTTGCATCACCCAGGGGA | BC020994 | 1229-1250 |
| Primer reverse | CTCACAGGCGCTGACCCCATAGT | S50916 | 180-158 |
| Sensor | TGAAGAGATAGTGCCCAGCCCTCCCTC-FL | S50916 | 66-92 |
| Anchor | LCRed640-CCACCCCCTCTACCCCGCATCTACA-P | S50916 | 94-118 |
Target . | Primer and hyridization probes 5′→3′ . | GenBank accession no. . | Position . |
|---|---|---|---|
| AML1-ETO | |||
| Primer forward | GAGGGAAAAGCTTCACTCTG | HS-AML1M | 1993-2012 |
| Primer reverse | TCGGGTGAAATGTCATTGCC | HSETO | 340-321 |
| Sensor | CCCTCACCACCCAATGGCTTCAGC-FL | HSETO | 183-206 |
| Anchor | LCRed640-TGGGCCTTCCTCTTCTTCCTCCTCC-P | HSETO | 209-234 |
| CBFB-MYH11 | |||
| Primer forward | GCAGGCAAGGTATATTTGAAGG | HUMCBFB | 253-274 |
| Primer reverse type A | CTCTTCTCCTCATTCTGCTC | HSSMMHC | 1500-1481 |
| Primer reverse type B | TTGAGCATTTTGTTGGTCCG | HSSMMHC | 1257-1238 |
| Primer reverse type C | CTTCCAGAGCTTCCACGGT | HSSMMHC | 902-884 |
| Primer reverse type D | TCCCTGTGACGCTCTCAACT | HSSMMHC | 611-592 |
| Primer reverse type E | GGCCAGGTCTGCGTTCTCT | HSSMMHC | 439-421 |
| Primer reverse type F | TCCTCTTCTCCTCATTCTGCTC | HSSMMHC | 1502-1481 |
| Sensor | GGCTCGCTCCTCATCAAACTCCA-FL | HUMCBFB | 375-353 |
| Anchor | LCRed640-ACAGCCCATACCATCCAGTCTTTGG-P | HUMCBFB | 351-327 |
| PML-RARA | |||
| Primer bcr1 (L-type) forward | GTCTTCCTGCCCAACAGCAACC | HUMPML3 | 1623-1644 |
| Primer bcr3 (S-type) forward | AGCTCTTGCATCACCCAGGGGA | BC020994 | 1229-1250 |
| Primer reverse | CTCACAGGCGCTGACCCCATAGT | S50916 | 180-158 |
| Sensor | TGAAGAGATAGTGCCCAGCCCTCCCTC-FL | S50916 | 66-92 |
| Anchor | LCRed640-CCACCCCCTCTACCCCGCATCTACA-P | S50916 | 94-118 |