Table 2. LightCycler conditions for the... | American Society of Hematology

Table 2.

LightCycler conditions for the detection of the different fusion cDNAs

		Segment 2					Segment 3
Temperature targets	Segment 1	*AML1-ETO*	*CBFB-MYH11*	*PML-RARA*	*AML1-ETO*	*CBFB-MYH11*	*PML-RARA*
Target temperature, °C	95	64	64	60	72	72	72
Incubation time, s	10	10	10	10	26	26	30
Temperature transition	20	20	20	20	2	20	2
Secondary target, °C	0	0	0	0	0	0	0
Step size, °C	0	0	0	0	0	0	0
Step delay, cycles	0	0	0	0	0	0	0
Acquisition	None	Single	Single	Single	None	None	None

		Segment 2					Segment 3
Temperature targets	Segment 1	*AML1-ETO*	*CBFB-MYH11*	*PML-RARA*	*AML1-ETO*	*CBFB-MYH11*	*PML-RARA*
Target temperature, °C	95	64	64	60	72	72	72
Incubation time, s	10	10	10	10	26	26	30
Temperature transition	20	20	20	20	2	20	2
Secondary target, °C	0	0	0	0	0	0	0
Step size, °C	0	0	0	0	0	0	0
Step delay, cycles	0	0	0	0	0	0	0
Acquisition	None	Single	Single	Single	None	None	None

These data are accumulated over 45 cycles in the quantification analysis mode.