DNA primers
Oligonucleotide . | Strand . | Corresponding sequence . |
|---|---|---|
| SPK89A 5′-GCCCATTACAATACAGAATGGAATC-3′ | Sense | 99 nt in intron before exon 10 (aa 358) |
| SPK89D 5′-AGGCTCTTCTCTGATCAGTGTGTGC-3′ | Antisense | Coding sequence for aa 496-502 |
| SPK89E 5′-CGGGATCCCATGGTCATGGTCACGGTCGTC-3′ | Sense | Coding aa 477-483 |
| SPK89F 5′-CGGAATTCGCATTTGTAAAGATTCCTACGCTG-3′ | Antisense | Corresponding to 31 nt into 3′nTR |
| SPK89G 5′-GCGAGATCTACATCAGTGATAAGA-3′ | Sense | Coding sequencing aa 358-363 |
Oligonucleotide . | Strand . | Corresponding sequence . |
|---|---|---|
| SPK89A 5′-GCCCATTACAATACAGAATGGAATC-3′ | Sense | 99 nt in intron before exon 10 (aa 358) |
| SPK89D 5′-AGGCTCTTCTCTGATCAGTGTGTGC-3′ | Antisense | Coding sequence for aa 496-502 |
| SPK89E 5′-CGGGATCCCATGGTCATGGTCACGGTCGTC-3′ | Sense | Coding aa 477-483 |
| SPK89F 5′-CGGAATTCGCATTTGTAAAGATTCCTACGCTG-3′ | Antisense | Corresponding to 31 nt into 3′nTR |
| SPK89G 5′-GCGAGATCTACATCAGTGATAAGA-3′ | Sense | Coding sequencing aa 358-363 |
The 531-bp PCR product coding for the human HK protein sequence, aa 358-502, was generated with SPK89A and SPK89D. The 492-bp PCR product coding for the HK protein sequence, aa 478-621, was generated with SPK89E and SPK89F. The 819-bp PCR product was generated using SPK89F and SPK89G.32 3′nTR indicates 3′N-terminal region.