Circulating platelets bound to B hermsii are not activated
. | . | Expression . | . | |
|---|---|---|---|---|
. | Platelets bound, % . | P-selectin . | β3-integrin . | |
| PMA, positive control | NA | 1.00 | 1.00 | |
| No agonist, negative control | NA | 0.07 | 0.48 | |
| DAH-bound platelets | 4.0 | 0.05 | 0.57 | |
| HS1-bound platelets | 4.6 | 0.05 | 0.57 | |
. | . | Expression . | . | |
|---|---|---|---|---|
. | Platelets bound, % . | P-selectin . | β3-integrin . | |
| PMA, positive control | NA | 1.00 | 1.00 | |
| No agonist, negative control | NA | 0.07 | 0.48 | |
| DAH-bound platelets | 4.0 | 0.05 | 0.57 | |
| HS1-bound platelets | 4.6 | 0.05 | 0.57 | |
Bacteria-bound platelets from the blood of mice infected with B hermsii strain DAH or HS1 were quantitated and analyzed for the expression of P-selectin or β3-integrin as described in “Materials and methods.” P-selectin and β3-integrin levels are expressed relative to the expression level of PMA-activated platelets, which was defined as 1.00. Shown are representative results of at least 2 independent experiments. NA indicates not applicable.