Quantification of erythroid colonies formed by different erythroblast populations
Cell type . | Primitive BFU-Es per 105 nucleated cells . | Mature BFU-Es . | CFU-Es per 105 nucleated cells . |
|---|---|---|---|
| Total FLCs | 67.5 ± 8 | Present | 7420 ± 510 |
| Fractionated FLCs | |||
| R1 | 15 ± 5 | Present | 41 200 ± 8800 |
| R2 | 0 | 0 | 3000 ± 50 |
| R3 | 0 | 0 | 0 |
| R4 | 0 | 0 | 0 |
| R5 | 0 | 0 | 0 |
Cell type . | Primitive BFU-Es per 105 nucleated cells . | Mature BFU-Es . | CFU-Es per 105 nucleated cells . |
|---|---|---|---|
| Total FLCs | 67.5 ± 8 | Present | 7420 ± 510 |
| Fractionated FLCs | |||
| R1 | 15 ± 5 | Present | 41 200 ± 8800 |
| R2 | 0 | 0 | 3000 ± 50 |
| R3 | 0 | 0 | 0 |
| R4 | 0 | 0 | 0 |
| R5 | 0 | 0 | 0 |
Total fetal liver cells (FLCs) were isolated from E14.5 embryos. Erythroblasts were double stained for CD71 and TER119, and R1-R5 cells were sorted by FACS as described in Figure 1. Results are presented as the number of colonies per 105 nucleated cells. The numbers are averages from duplicate cultures of 3 independent experiments. FLC indicates fetal liver cell.