Comparison of 125I-Epo binding to purified fractions of E14.5 fetal liver cells
Cell type . | Specific binding, fmol 125I-Epo/106 cells, average ± SD . | % of Ba/F3-EpoR cell-specific binding . | Estimated no. Epo-binding sites per cell . |
|---|---|---|---|
| Cultured Ba/F3-EpoR | 1.00 ± 0.24 | 100 | 1800 |
| Fetal liver* | |||
| R1 + R2 | 1.07 ± 0.31 | 107 | 1900 |
| R3 | 0.41 ± 0.10 | 41 | 750 |
| R4 | 0.14 ± 0.10 | 14 | 250 |
Cell type . | Specific binding, fmol 125I-Epo/106 cells, average ± SD . | % of Ba/F3-EpoR cell-specific binding . | Estimated no. Epo-binding sites per cell . |
|---|---|---|---|
| Cultured Ba/F3-EpoR | 1.00 ± 0.24 | 100 | 1800 |
| Fetal liver* | |||
| R1 + R2 | 1.07 ± 0.31 | 107 | 1900 |
| R3 | 0.41 ± 0.10 | 41 | 750 |
| R4 | 0.14 ± 0.10 | 14 | 250 |
SD indicates standard deviation.
Binding to fetal liver R1 plus R2 cells was determined by normalizing the specific 125I-Epo binding to purified Ter-119- cells with the percentage of R1 plus R2 cells present in the population (Figure 4, day 0)