Table 2. Genes differentially regulated... | American Society of Hematology

Table 2.

Genes differentially regulated upon exposure to Epo/SCF/Dex or Epo/SCF/ZK for 2, 6, 18, or 24 hours, including their regulation in GR^dim/dim versus wt erythroid progenitors

			2 hours			6 hours			18 hours			24 hours
Accession no.	Gene description	Frequency	fc	SD	fc	SD	Fc	SD	fc	SD	fc	SD
Dex up-regulated
*NM_011269*	*Rhesus blood group-associated A glycoprotein*	48	*1.08*	*0.27*	*2.02*	*0.19*	*1.26*	*0.29*	*1.77*	*0.48*	*0.91*	*0.36*
NM_011157	Proteoglycan, secretory granule	19	1.03	0.26	0.93	0.08	1.45	0.22	1.95	0.37	1.21	0.25
*BC006834*	*B-cell translocation gene 1, antiproliferative*	18	*0.98*	*0.19*	*2.41*	*0.28*	*3.35*	*2.22*	*1.29*	*0.27*	*0.67*	*0.15*
NM_009735	Beta-2 microglobulin (B2m)	21	0.99	0.18	1.34	0.12	1.92	0.43	2.48	0.95	0.65	0.15
*NM_018813*	*Cleavage and polyadenylation specificity factor 3*	11	*0.97*	*0.14*	*1.06*	*0.10*	*2.78*	*1.35*	*2.45*	*0.94*	*0.70*	*0.15*
NM_010634	Fatty acid binding protein 5, epidermal	10	1.04	0.26	0.89	0.07	1.73	0.41	3.17	1.57	0.84	0.19
*NM_008529*	*Lymphocyte antigen 6 complex, locus E*	10	*1.00*	*0.17*	*1.15*	*0.11*	*2.00*	*0.61*	*2.48*	*0.98*	*0.76*	*0.15*
AK003942	RIKEN:1110028E10	7	1.05	0.15	1.61	0.12	3.78	1.86	3.19	1.44	0.50	0.09
*AK020317*	*RIKEN:9230106B05*	6	*1.17*	*0.18*	*2.27*	*0.26*	*1.58*	*0.66*	*1.73*	*0.69*	*0.72*	*0.20*
NM_023719	Thioredoxin-interacting protein	4	1.16	0.27	1.82	0.17	1.08	0.24	1.21	0.44	1.10	0.33
*NM_013627*	*Paired box gene 6 (Pax6), mRNA*	4	*1.10*	*0.19*	*1.84*	*0.18*	*1.07*	*0.24*	*0.96*	*0.16*	*0.84*	*0.19*
NM_008722	Nucleophosmin 1 (Npm1)	3	0.99	0.26	0.75	0.14	1.68	0.34	3.28	1.46	0.75	0.14
*AF260927*	*D4Cole 1e*	2	*1.32*	*0.25*	*2.07*	*0.25*	*2.93*	*2.61*	*3.65*	*1.13*	*0.96*	*0.23*
NM_011755	Zinc finger protein 35	2	1.09	0.28	1.15	0.11	1.30	0.37	1.97	0.34	0.69	0.09
*NM_009429*	*Tumor protein, translationally controlled 1 (Tpl1)*	2	*1.06*	*0.34*	*0.98*	*0.05*	*1.32*	*0.18*	*2.26*	*0.61*	*0.66*	*0.21*
NM_011050	Programmed cell death 4 (Pdcd4)	2	0.98	0.31	0.85	0.09	1.57	0.28	2.37	0.70	0.65	0.15
*AW912233*	*Soares mammary gland, IMAGE:1514107 5′*	2	*1.05*	*0.15*	*1.25*	*0.12*	*2.20*	*0.24*	*3.75*	*2.01*	*0.55*	*0.25*
NM_008379	Karyopherin (importin) beta 1	2	0.90	0.13	0.86	0.17	1.41	0.13	2.55	1.00	1.07	0.29
Dex repressed
*AB020015*	*Beta-1-globin*	98	*1.05*	*0.25*	*0.84*	*0.18*	*0.67*	*0.24*	*0.62*	*0.20*	*1.57*	*0.39*
NM_009414	Tryptophan hydroxylase	30	1.44	0.33	0.64	0.30	1.30	0.42	1.71	0.57	6.37	2.66
*NM_016633*	*Erythroid differentiatiation factor*	15	*0.99*	*0.21*	*0.59*	*0.06*	*0.65*	*0.11*	*0.87*	*0.32*	*1.42*	*0.37*
NM_009801	Carbonic anhydrase 2 (Car2)	4	1.01	0.24	0.65	0.01	0.85	0.08	1.22	0.56	1.76	0.41

			2 hours			6 hours			18 hours			24 hours
Accession no.	Gene description	Frequency	fc	SD	fc	SD	Fc	SD	fc	SD	fc	SD
Dex up-regulated
*NM_011269*	*Rhesus blood group-associated A glycoprotein*	48	*1.08*	*0.27*	*2.02*	*0.19*	*1.26*	*0.29*	*1.77*	*0.48*	*0.91*	*0.36*
NM_011157	Proteoglycan, secretory granule	19	1.03	0.26	0.93	0.08	1.45	0.22	1.95	0.37	1.21	0.25
*BC006834*	*B-cell translocation gene 1, antiproliferative*	18	*0.98*	*0.19*	*2.41*	*0.28*	*3.35*	*2.22*	*1.29*	*0.27*	*0.67*	*0.15*
NM_009735	Beta-2 microglobulin (B2m)	21	0.99	0.18	1.34	0.12	1.92	0.43	2.48	0.95	0.65	0.15
*NM_018813*	*Cleavage and polyadenylation specificity factor 3*	11	*0.97*	*0.14*	*1.06*	*0.10*	*2.78*	*1.35*	*2.45*	*0.94*	*0.70*	*0.15*
NM_010634	Fatty acid binding protein 5, epidermal	10	1.04	0.26	0.89	0.07	1.73	0.41	3.17	1.57	0.84	0.19
*NM_008529*	*Lymphocyte antigen 6 complex, locus E*	10	*1.00*	*0.17*	*1.15*	*0.11*	*2.00*	*0.61*	*2.48*	*0.98*	*0.76*	*0.15*
AK003942	RIKEN:1110028E10	7	1.05	0.15	1.61	0.12	3.78	1.86	3.19	1.44	0.50	0.09
*AK020317*	*RIKEN:9230106B05*	6	*1.17*	*0.18*	*2.27*	*0.26*	*1.58*	*0.66*	*1.73*	*0.69*	*0.72*	*0.20*
NM_023719	Thioredoxin-interacting protein	4	1.16	0.27	1.82	0.17	1.08	0.24	1.21	0.44	1.10	0.33
*NM_013627*	*Paired box gene 6 (Pax6), mRNA*	4	*1.10*	*0.19*	*1.84*	*0.18*	*1.07*	*0.24*	*0.96*	*0.16*	*0.84*	*0.19*
NM_008722	Nucleophosmin 1 (Npm1)	3	0.99	0.26	0.75	0.14	1.68	0.34	3.28	1.46	0.75	0.14
*AF260927*	*D4Cole 1e*	2	*1.32*	*0.25*	*2.07*	*0.25*	*2.93*	*2.61*	*3.65*	*1.13*	*0.96*	*0.23*
NM_011755	Zinc finger protein 35	2	1.09	0.28	1.15	0.11	1.30	0.37	1.97	0.34	0.69	0.09
*NM_009429*	*Tumor protein, translationally controlled 1 (Tpl1)*	2	*1.06*	*0.34*	*0.98*	*0.05*	*1.32*	*0.18*	*2.26*	*0.61*	*0.66*	*0.21*
NM_011050	Programmed cell death 4 (Pdcd4)	2	0.98	0.31	0.85	0.09	1.57	0.28	2.37	0.70	0.65	0.15
*AW912233*	*Soares mammary gland, IMAGE:1514107 5′*	2	*1.05*	*0.15*	*1.25*	*0.12*	*2.20*	*0.24*	*3.75*	*2.01*	*0.55*	*0.25*
NM_008379	Karyopherin (importin) beta 1	2	0.90	0.13	0.86	0.17	1.41	0.13	2.55	1.00	1.07	0.29
Dex repressed
*AB020015*	*Beta-1-globin*	98	*1.05*	*0.25*	*0.84*	*0.18*	*0.67*	*0.24*	*0.62*	*0.20*	*1.57*	*0.39*
NM_009414	Tryptophan hydroxylase	30	1.44	0.33	0.64	0.30	1.30	0.42	1.71	0.57	6.37	2.66
*NM_016633*	*Erythroid differentiatiation factor*	15	*0.99*	*0.21*	*0.59*	*0.06*	*0.65*	*0.11*	*0.87*	*0.32*	*1.42*	*0.37*
NM_009801	Carbonic anhydrase 2 (Car2)	4	1.01	0.24	0.65	0.01	0.85	0.08	1.22	0.56	1.76	0.41

The ratio between Epo/SCF/Dex over Epo/SCF/ZK at any time point is given as fold change (fc). When the frequency of a sequence was 2 or more (n > 1) the data points were averaged and the standard deviation (SD) is shown. wt indicates wild type.

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