Epo, SCF, and Dex in the regulation of the erythroid gene expression program
. | Down-regulation . | . | . | Up-regulation . | . | . | . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
. | Fold change less than -2 . | Fold change -2.0 to -1.75 . | Fold change -1.75 to -1.50 . | Fold change 1.50 to 1.75 . | Fold change 1.75 to 2.0 . | Fold change 2.0 to 2.5 . | Fold change more than 2.5 . | |||||
| Epo, ‰ | 0.9 | 4.8 | 10.2 | 12.7 | 4.2 | 1.8 | 1.2 | |||||
| Dex, ‰ | 0.2 | 1.1 | 3.6 | 1.8 | 0.9 | 0.4 | 0.1 | |||||
| SCF, ‰ | 1.2 | 3.9 | 8.9 | 14.1 | 4.1 | 2.1 | 1.1 | |||||
| Ratio ESD/ESZK, ‰ | 0.2 | 1.2 | 4.1 | 5.9 | 1.8 | 0.8 | 0.3 | |||||
. | Down-regulation . | . | . | Up-regulation . | . | . | . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
. | Fold change less than -2 . | Fold change -2.0 to -1.75 . | Fold change -1.75 to -1.50 . | Fold change 1.50 to 1.75 . | Fold change 1.75 to 2.0 . | Fold change 2.0 to 2.5 . | Fold change more than 2.5 . | |||||
| Epo, ‰ | 0.9 | 4.8 | 10.2 | 12.7 | 4.2 | 1.8 | 1.2 | |||||
| Dex, ‰ | 0.2 | 1.1 | 3.6 | 1.8 | 0.9 | 0.4 | 0.1 | |||||
| SCF, ‰ | 1.2 | 3.9 | 8.9 | 14.1 | 4.1 | 2.1 | 1.1 | |||||
| Ratio ESD/ESZK, ‰ | 0.2 | 1.2 | 4.1 | 5.9 | 1.8 | 0.8 | 0.3 | |||||
Erythroid progenitors were factor deprived and restimulated with Epo, Dex, SCF, Epo/SCF, Epo/SCF/Dex, or Epo/SCF/ZK. Cy5-labeled cDNA synthesized from mRNA of restimulated cells was hybridized in parallel with Cy3-labeled cDNA synthesized from factor-deprived cells to the hematopoietic and the 17K EST arrays. Values in table represent the fraction (given in permillage [‰]) of cDNAs present on the microarrays that shows a certain fold change of expression in stimulated versus unstimulated cells.