Levels of γ-globin expression in the fetal liver (fl) and adult blood related to chromosomal integration sites in the single copy lines generated
. | % γ expression/βmaj per copy . | . | . | . | ||
|---|---|---|---|---|---|---|
| Line . | 12.5 d fl . | 16.5 d fl . | Adult blood . | Integration sites . | ||
| LCR Aγ | ||||||
| C | 8.6 | 2.6 | 0 | Euchromatic | ||
| G | 24.8 | 4.4 | 0.1 | Euchromatic | ||
| F | 26 | 7 | 0.14 | N. centromeric | ||
| HPFH-2 | ||||||
| A | 11.4 | 4.6 | 0.24 | Telomeric | ||
| C | 87.6 | 56 | 6 | Euchromatic | ||
| F | 44.6 | 10 | 0.72 | Euchromatic | ||
| HPFH-3 | ||||||
| A | 11.6 | 1.08 | 0 | Euchromatic | ||
| C | 2 | 0 | 0 | Euchromatic | ||
| HPFH-6 | ||||||
| A | 34 | 11.6 | 2 | Euchromatic | ||
| B | 11.8 | 0.12 | 0 | Telomeric | ||
| C | — | — | 0 | N. centromeric | ||
. | % γ expression/βmaj per copy . | . | . | . | ||
|---|---|---|---|---|---|---|
| Line . | 12.5 d fl . | 16.5 d fl . | Adult blood . | Integration sites . | ||
| LCR Aγ | ||||||
| C | 8.6 | 2.6 | 0 | Euchromatic | ||
| G | 24.8 | 4.4 | 0.1 | Euchromatic | ||
| F | 26 | 7 | 0.14 | N. centromeric | ||
| HPFH-2 | ||||||
| A | 11.4 | 4.6 | 0.24 | Telomeric | ||
| C | 87.6 | 56 | 6 | Euchromatic | ||
| F | 44.6 | 10 | 0.72 | Euchromatic | ||
| HPFH-3 | ||||||
| A | 11.6 | 1.08 | 0 | Euchromatic | ||
| C | 2 | 0 | 0 | Euchromatic | ||
| HPFH-6 | ||||||
| A | 34 | 11.6 | 2 | Euchromatic | ||
| B | 11.8 | 0.12 | 0 | Telomeric | ||
| C | — | — | 0 | N. centromeric | ||
N. centromeric indicates near centromeric integration; and —, not determined.