Transplantation and enrichment characteristics
. | Enrichment purity, % CD34 . | Fluorescence assignment (% purity) . | . | Cells to animal/arm . | ANC more than 500, day after transplantation . | |
|---|---|---|---|---|---|---|
. | . | Non-SAR . | SAR . | . | . | |
| M00081 | 87 | EGFP (98) | EYFP (97) | 2.3 × 107 | 28 | |
| J00116 | 77 | EYFP (89) | EGFP (95) | 1.8 × 107 | 29 | |
| T00024 | 90 | EGFP (97) | EYFP (93) | 1.6 × 107 | 27 | |
. | Enrichment purity, % CD34 . | Fluorescence assignment (% purity) . | . | Cells to animal/arm . | ANC more than 500, day after transplantation . | |
|---|---|---|---|---|---|---|
. | . | Non-SAR . | SAR . | . | . | |
| M00081 | 87 | EGFP (98) | EYFP (97) | 2.3 × 107 | 28 | |
| J00116 | 77 | EYFP (89) | EGFP (95) | 1.8 × 107 | 29 | |
| T00024 | 90 | EGFP (97) | EYFP (93) | 1.6 × 107 | 27 | |
Animals received 5 days of rhSCF (50 μg/kg per day) and rhG-CSF (100 μg/kg per day) subcutaneously (kindly provided by Graham Molineux, Amgen, Thousand Oaks, CA) before marrow harvest and myeloablative irradiation (total body irradiation, 1020 cGy). Animals received 100 μg/kg rhG-CSF intravenously, once daily from day 0 until their peripheral blood neutrophil counts were higher than 1 × 109/L (1000/μL). Engraftment defines the first day of a neutrophil recovery to higher than 0.5 × 109/L in the peripheral blood. The table denotes the composition of autologous EGFP/EYFP-marked graft products returned to animals M00081, T00024, and J00116 as noted. ANC indicates absolute neutrophil count; EGFP, enhanced green fluorescent protein; and EYFP, enhanced yellow fluorescent protein.