JAML expression enhances U937 cell adhesion to HBMECs
. | HBMECs . | HBMECs + TNFα . |
|---|---|---|
| U937/MT | ||
| - Zn | 1.00 ± 0.36 | 5.29 ± 0.17 |
| + Zn | 1.07 ± 0.33 | 4.79 ± 0.29 |
| U937/MT-JAML | ||
| - Zn | 1.00 ± 0.30 | 6.74 ± 0.52 |
| + Zn | 2.65 ± 0.17 | 11.04 ± 1.39 |
| U937/MT-JAMLK54D | ||
| - Zn | 1.00 ± 0.13 | 4.83 ± 0.30 |
| + Zn | 1.38 ± 0.47 | 4.23 ± 0.47 |
. | HBMECs . | HBMECs + TNFα . |
|---|---|---|
| U937/MT | ||
| - Zn | 1.00 ± 0.36 | 5.29 ± 0.17 |
| + Zn | 1.07 ± 0.33 | 4.79 ± 0.29 |
| U937/MT-JAML | ||
| - Zn | 1.00 ± 0.30 | 6.74 ± 0.52 |
| + Zn | 2.65 ± 0.17 | 11.04 ± 1.39 |
| U937/MT-JAMLK54D | ||
| - Zn | 1.00 ± 0.13 | 4.83 ± 0.30 |
| + Zn | 1.38 ± 0.47 | 4.23 ± 0.47 |
U937/MT, U937/MT-JAML-HA, and U937/MT-JAMLK54D-HA cells stably transfected with the pMT, the pMT-JAML-HA, or pMT-JAMLK54D-HA respectively were untreated (-Zn) or treated (+Zn) with ZnSO4 for 18 hours prior to fluorescent labeling and subjected to a cell adhesion assay using human bone marrow endothelial cells either untreated (HBMECs) or treated (HBMECs + TNFα) as a target layer. Results are expressed in each condition as the amount of released fluorescence from adherent cells, normalized to unity for the corresponding -Zn/-TNFα condition. The adhesion level of U937/MT-JAML-HA cells onto untreated or TNFα-pretreated HBMECs, in the absence of Zn treatment (-Zn), was measured as 2.3% and 15.5% respectively, of the total number of incubated cells. Values shown are ± SD.