Effect of DI-TSP peptide on FGF-2 binding to TSP-1 and on FGF-2 interaction with the matrix
Condition . | Concentration . | Inhibition of FGF-2 binding to TSP-1, % of control . | Inhibition of FGF-2 binding to ECM, % of control . | Mobilization of matrix-bound FGF-2, % of bound FGF-2 . |
|---|---|---|---|---|
| Vehicle | — | 100.0 ± 6.0 | 100.0 ± 3.3 | 29.6 ± 0.3 |
| TSP-1 | 100 nM | 13.7 ± 6.5 | 14.8 ± 2.3 | 74.0 ± 1.2 |
| DI-TSP | 10 μM | 90.0 ± 3.2 | 104.0 ± 6.4 | 30.4 ± 0.3 |
| DI-TSP | 1 μM | 84.0 ± 1.7 | 104.1 ± 1.0 | NT |
| DI-TSP | 100 nM | 87.0 ± 21.1 | 101.4 ± 6.4 | NT |
Condition . | Concentration . | Inhibition of FGF-2 binding to TSP-1, % of control . | Inhibition of FGF-2 binding to ECM, % of control . | Mobilization of matrix-bound FGF-2, % of bound FGF-2 . |
|---|---|---|---|---|
| Vehicle | — | 100.0 ± 6.0 | 100.0 ± 3.3 | 29.6 ± 0.3 |
| TSP-1 | 100 nM | 13.7 ± 6.5 | 14.8 ± 2.3 | 74.0 ± 1.2 |
| DI-TSP | 10 μM | 90.0 ± 3.2 | 104.0 ± 6.4 | 30.4 ± 0.3 |
| DI-TSP | 1 μM | 84.0 ± 1.7 | 104.1 ± 1.0 | NT |
| DI-TSP | 100 nM | 87.0 ± 21.1 | 101.4 ± 6.4 | NT |
Labeled FGF-2 was incubated with immobilized TSP-1 alone (vehicle) or in the presence of soluble TSP-1 or DI-TSP peptide, used as competitors, as described. 31 Data are the percentage of control binding, without competitors (vehicle). The ability of TSP-1 and DI-TSP peptide to affect FGF-2 binding to the matrix and to mobilize FGF-2 from the matrix were assayed as described in “Materials and methods.”—indicates not applicable; NT, not tested.