Effect of CTLs against kinectin-derived peptide on colony formation by CD34+ cells
Experiment . | CTLs against kinectin peptide . | Blocking MoAb . | CFU-GM . | BFU-E . |
|---|---|---|---|---|
| 1 | None | None | 48 ± 4 | 22 ± 2 |
| 645F | None | 26 ± 2 | 24 ± 4 | |
| 645F | mlgG2b | 25 ± 3 | 26 ± 3 | |
| 645F | BB7.2 | 48 ± 3 | 27 ± 2 | |
| 794S | None | 44 ± 3 | 25 ± 2 | |
| 794S | mlgG2b | 48 ± 3 | 25 ± 3 | |
| 794S | BB7.2 | 49 ± 4 | 24 ± 3 | |
| 2 | None | None | 64 ± 5 | 22 ± 2 |
| 645F | None | 36 ± 5 | 25 ± 2 | |
| 645F | mlgG2b | 38 ± 2 | 25 ± 3 | |
| 645F | BB7.2 | 68 ± 3 | 25 ± 3 | |
| 794S | None | 68 ± 3 | 24 ± 5 | |
| 794S | mlgG2b | 69 ± 5 | 24 ± 3 | |
| 794S | BB7.2 | 71 ± 5 | 24 ± 4 |
Experiment . | CTLs against kinectin peptide . | Blocking MoAb . | CFU-GM . | BFU-E . |
|---|---|---|---|---|
| 1 | None | None | 48 ± 4 | 22 ± 2 |
| 645F | None | 26 ± 2 | 24 ± 4 | |
| 645F | mlgG2b | 25 ± 3 | 26 ± 3 | |
| 645F | BB7.2 | 48 ± 3 | 27 ± 2 | |
| 794S | None | 44 ± 3 | 25 ± 2 | |
| 794S | mlgG2b | 48 ± 3 | 25 ± 3 | |
| 794S | BB7.2 | 49 ± 4 | 24 ± 3 | |
| 2 | None | None | 64 ± 5 | 22 ± 2 |
| 645F | None | 36 ± 5 | 25 ± 2 | |
| 645F | mlgG2b | 38 ± 2 | 25 ± 3 | |
| 645F | BB7.2 | 68 ± 3 | 25 ± 3 | |
| 794S | None | 68 ± 3 | 24 ± 5 | |
| 794S | mlgG2b | 69 ± 5 | 24 ± 3 | |
| 794S | BB7.2 | 71 ± 5 | 24 ± 4 |
Representative results of 3 similar experiments performed in an at least sextuplicated manner. Data are represented as means ± SD. BB7.2 indicates HLA-A2-specific MoAb; mlgG2b, isotype control.